Processing of a sample fluid with target components

1. A cartridge for the detection of target components (T 1 , T 2 ) in a sample fluid with magnetic particles (MP 1 , MP 2 , MP), comprising:

a reaction chamber in which the sample fluid can be provided and which has a reaction surface;

at least one investigation region that is located on the reaction surface and is at least partially bordered by a physical barrier;

wherein the investigation region is subdivided into two concentric subregions;

wherein reagents that specifically bind to target components are deposited in one of the subregions and the magnetic particles are deposited in the other subregion;

wherein the reaction surface is hydrophilic outside the investigation region;

a magnetic field generator for generating a magnetic field (B) that confines the magnetic particles (MP 1 , MP 2 , MP) at an associated investigation region during introduction of the sample fluid into the reaction chamber;

an optical, magnetic, mechanical, acoustic, thermal or electrical sensor unit for the detection of target components (T 1 , T 2 ) in the investigation region;

wherein the physical barrier has a height (h) of 1 μm to 100 μm and a width (w) of 2 μm to 500 μm and is configured not to impede a lateral movement of the magnetic particles (MP 1 , MP 2 , MP).

2. A cartridge for the processing of a sample fluid with target components (T 1 , T 2 ), comprising:

a reaction chamber in which the sample fluid can be provided and which has a reaction surface;

at least one investigation region that is located on the reaction surface, is at least partially bordered by a physical barrier, and comprises reagents, for example capture probes (CP 1 , CP 2 ), that specifically bind target components (T 1 , T 2 ) of the sample fluid;

wherein the reaction surface is hydrophilic outside the investigation region;

wherein the physical barrier has a height (h) of 1 μm to 100 μm and a width (w) of 2 μm to 500 μM and is configured not to impede a lateral movement of the magnetic particles (MP 1 , MP 2 , MP); and

wherein the investigation region is divided into two concentric subregions, the reagents being deposited in one of the subregions and the magnetic particles being deposited in the other.

3. A method for manufacturing a cartridge according to claim 1 , said method comprising the following steps:

providing a reaction chamber with a reaction surface and with at least one investigation region that is at least partially bordered by a physical barrier, the investigation region being subdivided into concentric subregions;

wherein the reaction surface is hydrophilic outside the investigation region; and wherein the physical barrier has a height (h) of 1 μm to 100 μm and a width (w) of 2 μm to 500 μm and is configured not to impede a lateral movement of the magnetic particles (MP 1 , MP 2 , MP); and

depositing reagents like capture probes (CP 1 , CP 2 ) in the one of the subregions.

4. A method for the detection of target components (T 1 , T 2 ) in a sample fluid, said method comprising the following steps:

introducing the sample fluid into the reaction chamber of a cartridge according to claim 1 ;

detecting target components (T 1 , T 2 ) bound to capture probes (CP 1 , CP 2 ) in the investigation region.

5. The method according to claim 4 , wherein the detection is done by an optical, magnetic, mechanical, acoustic, thermal or electrical procedure.

6. The cartridge according to claim 1 , wherein the physical barrier comprises a protrusion and/or a wall of a recess or an elevation.

7. The cartridge according to claim 1 , wherein the reaction surface is hydrophobic inside the investigation region.

8. The cartridge according to claim 1 , wherein the reagents include capture probes (CP 1 , CP 2 , CP) which specifically bind to at least one of nucleic acids, proteins, antigens, ligands, lipids, drugs, vitamins, hormones, haptens, carbohydrates, cellular fragments, and related compounds, including troponin I, myoglobin, B-type natriuretic peptide, and/or 2,3 C-reactive protein.

9. The cartridge according to claim 1 , wherein the magnetic particles (MP 1 , MP 2 , MP) are added to the sample fluid.

10. The cartridge according to claim 8 , wherein the magnetic particles (MP 1 , MP 2 , MP) are confined in the other of the subregions by the magnetic field (B).

11. The cartridge according to claim 8 , wherein the magnetic particles (MP 1 , MP 2 , MP) are disposed in the other of the subregions.

12. The cartridge according to claim 1 , wherein the reaction chamber comprises at least two subregions

with different capture probes (CP 1 , CP 2 ) that are specific for different target components (T 1 , T 2 ) and/or

with different types of magnetic particles (MP 1 , MP 2 ).

13. The cartridge according to claim 1 , wherein the physical barrier has a height (h) of about 10 μm.

14. The cartridge according to claim 13 , wherein the physical barrier has a width (w) of about 20 μm.

15. A cartridge for detection of target components (T 1 , T 2 ) in a sample fluid with magnetic particles (MP 1 , MP 2 , MP), comprising:

a reaction chamber in which the sample fluid can be provided and which has a reaction surface;

at least one investigation region that is located on the reaction surface and is at least partially bordered by a physical barrier;

wherein the reaction surface is hydrophilic outside the investigation region;

a magnetic field generator for generating a magnetic field (B) that confines the magnetic particles (MP 1 , MP 2 , MP) at an associated investigation region during introduction of the sample fluid into the reaction chamber;

an optical, magnetic, mechanical, acoustic, thermal or electrical sensor unit for the detection of target components (T 1 , T 2 ) in the investigation region;

wherein the physical barrier has a height (h) of 1 μm to 100 μm and a width (w) of 2 to 500 μm and is configured not to impede a lateral movement of the magnetic particles (MP 1 , MP 2 , MP),

wherein the at least one investigation region includes first and second concentric subregions and wherein a portion of the physical barrier is disposed between the subregions, surrounding the inner subregion and another portion of the physical barrier surrounds an outer of the concentric subregions;

wherein reagents that specifically bind to target components are deposited in at least one of the subregions.