Pathways to adipate semialdehyde and other organic products
Recombinant microorganisms comprising at least one exogenous nucleic acid sequence and capable of producing adipate semialdehyde are provided. Adipate semialdehyde may be produced in a synthesis pathway utilizing a single thiolase reaction. Adipate semialdehyde may also be produced from intermediates consisting of alpha, omega difunctional aliphatic organic molecules. Methods of using recombinant microorganisms to produce 6-aminocaproic acid, adipic acid, hexamethylenediamine and 1.6-hexanediol are also provided.
1. A recombinant microorganism genetically engineered to produce adipate semialdehyde from 4-hydroxybutyryl-CoA, the recombinant microorganism comprising a first exogenous nucleic acid encoding a CoA synthase of class E.C. 2.3.1.X that catalyzes a substrate to product conversion of malonyl-CoA and 4-hydroxybutyryl-CoA to 6-hydroxy-3-oxo-hexanoyl-CoA and CO 2 , wherein the recombinant microorganism produces adipate semialdehyde from 4-hydroxybutyryl-CoA via the intermediates: 6-hydroxy-3-oxo-hexanoyl-CoA; 3,6-dihydroxyhexanoyl-CoA; 6-hydroxy-2-hexenoyl-CoA; 6-hydroxyhexanoyl-CoA; and 6-hydroxycaproic acid.
2. The recombinant microorganism of claim 1 , wherein the CoA synthase decarboxylase is a CoA synthase decarboxylase of class E.C. 2.3.1.165, E.C. 2.3.1.194, E.C. 2.3.1.199, E.C. 2.3.1.206, or E.C. 2.3.1.221.
3. The recombinant microorganism of claim 1 , further comprising at least a second exogenous nucleic acid encoding at least one further enzyme selected from an acetyltransferase (E.C. 2.3.1.X), ACP synthase decarboxylase (E.C. 2.3.1.X), 3-oxoCoA dehydrogenase (1.1.1.X), enolyl-CoA dehydratase (E.C. 4.2.1.X), acrylyl-CoA reductase (E.C. 1.3.1.X), acyl-CoA dehydrogenase (E.C. 1.3.8.X), CoA transferase (E.C. 2.8.3.X), thioesterase (E.C. 3.1.2.X), dehydrogenase (E.C. 1.1.1.X), alcohol dehydrogenase (E.D. 1.1.1.X), and transaminase (E.C. 2.6.1.X).
4. The recombinant microorganism of claim 3 , further comprising a third exogenous nucleic acid encoding an enzyme that converts 6-hydroxycaproic acid to adipate semialdehyde.
5. The recombinant microorganism of claim 4 , further comprising a fourth exogenous nucleic acid encoding an enzyme that converts adipate semialdehyde to an organic molecule selected from 6-aminocaproic acid, adipatic acid, hexamethylenediamine, and 1,6-hexanediol.
6. The recombinant microorganism of claim 1 wherein the recombinant microorganism is a yeast.
7. The recombinant microorganism of claim 1 wherein the recombinant microorganism is a bacteria.
8. The recombinant microorganism of claim 1 wherein the aqueous solubility of each of the intermediates is greater than 20 mg/mL.
9. The recombinant microorganism of claim 3 , wherein the recombinant microorganism comprises exogenous nucleic acids encoding at least three of said further enzymes.
10. A recombinant microorganism genetically engineered to produce adipate semialdehyde from 4-hydroxybutyryl-CoA, the recombinant microorganism comprising a first exogenous nucleic acid encoding an enzyme of class E.C. 2.3.1.165, E.C. 2.3.1.194, E.C. 2.3.1.199, E.C. 2.3.1.206, or E.C. 2.3.1.221 that catalyzes a substrate to product conversion of malonyl-CoA and 4-hydroxybutyryl-CoA to 6-hydroxy-3-oxo-hexanoyl-CoA and CO 2 , and wherein the recombinant microorganism produces adipate semialdehyde from 4-hydroxybutyryl-CoA via the intermediates: 6-hydroxy-3-oxo-hexanoyl-CoA; 3,6-dihydroxyhexanoyl-CoA; 6-hydroxy-2-hexenoyl-CoA; 6-hydroxyhexanoyl-CoA; and 6-hydroxycaproic acid.
11. The recombinant microorganism of claim 10 further comprising a second exogenous nucleic acid encoding an enzyme of class E.C. 2.3.1.9, E.C. 2.3.1.174, or E.C. 2.3.1.223 that catalyzes a substrate to product conversion of acetyl-CoA and 4-hydroxybutyryl-CoA to 6-hydroxy-3-oxo-hexanoyl-CoA, and/or a third exogenous nucleic acid encoding an enzyme of class E.C. 2.3.1.41, E.C. 2.3.1.179, or E.C. 2.3.1.180 that catalyzes a substrate to product conversion of malonyl-ACP and 4-hydroxybutyryl-CoA to 6-hydroxy-3-oxo-hexanoyl-CoA and CO 2 .