Immunogenic composition of killed leptospira bacteria
The present invention pertains to a composition containing an immunogenic cell preparation of killed Leptospira bacteria in an ethylenediaminetetraacetic acid solution. The invention also pertains to a vaccine to protect an animal against an infection with leptospira bacteria, wherein the vaccine comprises this composition. Also, the invention pertains to the use of ethylenediaminetetraacetic acid to stabilize an immunogenic preparation of killed Leptospira bacteria in a liquid carrier, by dissolving the ethylenediaminetetraacetic acid in the carrier.
1. A composition comprising an immunogenic cell preparation of killed whole cell Leptospira bacteria and 5 to 50 mmols of ethylenediaminetetraacetic acid (EDTA) per liter solution; wherein the killed whole cell Leptospira bacteria are Leptospira interrogans serogroup Australis serovar Bratislava.
2. The composition of claim 1 , wherein the solution comprises 20 mmols of ethylenediaminetetraacetic acid per liter titre.
3. The composition of claim 1 , wherein the solution contains dibasic sodium phosphate.
4. The composition of claim 2 , wherein the solution contains dibasic sodium phosphate.
5. A vaccine to protect an animal against an infection with Leptospira bacteria, wherein the vaccine comprises the composition of claim 1 .
6. A vaccine to protect an animal against an infection with Leptospira bacteria, wherein the vaccine comprises the composition of claim 2 .
7. A vaccine to protect an animal against an infection with Leptospira bacteria, wherein the vaccine comprises the composition of claim 3 .
8. A vaccine to protect an animal against an infection with Leptospira bacteria, wherein the vaccine comprises the composition of claim 4 .