Salmonid alphavirus and uses thereof
View Patent ↗This disclosure relates to reagents, methods for treating, diagnosing, and tracking diseases associated with salmon alphavirus.
1. A deoxyribonucleic acid (DNA) expression vector encoding a salmon alphavirus (SAV) polyprotein; wherein said SAV polyprotein is at least 98% identical with SEQ ID NO: 5.
2. The DNA expression vector of claim 1 , comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3.
3. A DNA expression vector encoding a SAV polyprotein comprising the sequence of SEQ ID NO: 5.
4. A method for inducing an immune response in a host against a salmon alphavirus comprising administering to the host a DNA expression vector encoding a SAV polyprotein; wherein said SAV polyprotein is at least 98% identical with SEQ ID NO:5.
5. A method for inducing an immune response in a host against a salmon alphavirus comprising administering to the host a polypeptide or peptides sharing at least 98% identity with SEQ ID NO: 5.
6. The method of claim 4 , wherein said DNA expression vector is a plasmid which is administered by injection into muscle tissue.
7. The method of claim 4 , wherein two to 20 micrograms of said DNA expression vector is administered to the host.
8. A vaccine comprising the DNA expression vector of claim 1 .
9. The method of claim 7 , wherein 5 to 10 micrograms of the DNA expression vector is administered to the host.
10. The method of claim 4 , wherein the DNA expression vector is a supercoiled plasmid; and wherein 5 to 10 micrograms of the DNA expression vector is administered to the host by injection into muscle tissue.
11. A method for inducing an immune response in a host against a salmon alphavirus comprising administering to the host a DNA expression vector encoding a SAV polyprotein; wherein said expression vector comprises a sequence selected from the group of SEQ ID NO:1, SEQ ID NO: 2, and SEQ ID NO: 3.
12. The method of claim 11 , wherein the DNA expression vector is a supercoiled plasmid; and wherein 5 to 10 micrograms of the DNA expression vector is administered to the host by injection into muscle tissue.
13. A vaccine comprising the DNA expression vector of claim 2 .
14. A method for inducing an immune response in a host against a salmon alphavirus comprising administering to the host a DNA expression vector encoding a SAV polyprotein comprising the sequence of SEQ ID NO: 5.
15. The method of claim 14 , wherein the DNA expression vector is a supercoiled plasmid; and wherein 5 to 10 micrograms of the DNA expression vector is administered to the host by injection into muscle tissue.
16. A vaccine comprising the DNA expression vector of claim 3 .