Method for selection of agents influencing intestinal motility disorders and pain
A method is provided for evaluating agents for the treatment of different intestinal motility disorders, using distinct methodological parts related to musculature and nerves of the GI tract which communicate with the brain. In particular, the present invention provides a method for the selection of an agent effective for the treatment of an intestinal motility disorder, wherein said method comprises: a) a step of spatiotemporal (ST) mapping carried out on a gastrointestinal segment to analyze the effect of said agent on gastrointestinal motility; and b) a step of ex vivo nerve bundle recording carried out on a gastrointestinal segment to analyze the effect of said agent on mesenteric afferent nerve firing. Bacterial strains selected by the methods of the invention and the use of said bacterial strains in the treatment of intestinal motility disorders are also provided.
1. A method of treating an intestinal motility disorder in a subject in need thereof comprising: administering a bacterial strain to said subject in an amount effective to treat said intestinal motility disorder, the bacterial strain selected by a method of a) a step of spatiotemporal (ST) mapping carried out on a gastrointestinal segment to analyse the effect of said bacterial strain on gastrointestinal motility; and b) a step of ex vivo nerve bundle recording carried out on a gastrointestinal segment to analyse the effect of said bacterial strain on mesenteric afferent nerve firing, wherein the bacterial strain is a probiotic bacterial strain.
2. The method of claim 1 , wherein step a) further comprises the measurement of intraluminal pressure.
3. The method of claim 1 , wherein step b) comprises measuring the spontaneous firing frequencies of mesenteric afferent nerve bundles.
4. The method of claim 1 , wherein said gastrointestinal segment is an ex vivo segment of jejunum or colon.
5. The method of claim 1 , wherein the intestinal motility disorder is one in which it is desired to reduce the transit time of material through the gastrointestinal tract and the probiotic bacterial strain shows the ability to increase gastrointestinal motility and to decrease mesenteric afferent nerve firing.
6. The method of claim 1 , wherein the probiotic bacterial strain shows the ability to increase migrating motor complex (MMC) frequency or increase MMC velocity or increase intraluminal pressure, and the bacterial strain shows the ability to decrease pain signalling.
7. The method of claim 1 , wherein the intestinal motility disorder is one in which it is desired to increase the transit time of material through the gastrointestinal tract, and the probiotic bacterial strain shows an ability to decrease gastrointestinal motility and to decrease mesenteric afferent nerve firing.
8. The method of claim 7 , wherein the probiotic bacterial strain shows an ability to decrease MMC frequency or decrease MMC velocity or decrease intraluminal pressure, and the bacterial strain shows an ability to decrease pain signalling.
9. The method of claim 1 , wherein the probiotic bacterial strain is LG345A (DSMZ Depository No. DSM 27123) or LG621A (DSMZ Depository No. DSM 27126).
10. The method of claim 1 , wherein said intestinal motility disorder is constipation or colic.
11. The method of claim 1 , wherein said intestinal motility disorder is infantile colic.
12. The method of claim 10 , wherein the probiotic bacterial strain is LG345A (DSMZ Depository No. DSM 27123).
13. The method of claim 11 , wherein the probiotic bacterial strain is LG345A (DSMZ Depository No. DSM 27123).
14. The method of claim 1 , the intestinal motility disorder is irritable bowel syndrome.
15. The method of claim 14 , wherein the probiotic bacterial strain is LG621A (DSMZ Depository No. DSM 27126).