Drug delivery composition comprising proteins and biodegradable polyesteramides
View Patent ↗Drug delivery compositions are provided having proteins and biodegradable polymers that may be used for controlled and long term release of proteins into a biological environment. According to some embodiments, the drug delivery composition may be provided with at least a protein and a biodegradable polymer possessing at least two different functional groups selected from the group of chosen among carboxyl, ester, amine, amide, thiol, thioester or hydroxyl groups pendant to the main polymer chain whereby the composition absorbs between 5-10% w/w water when exposed to physiological conditions for at least 20 days. A drug delivery system for controlled protein release which includes the drug delivery composition is also provided whereby the drug delivery system may be in the form of microparticles, films, coatings, fibers, pellets, cylinders, discs, implants, microcapsules, microspheres, nanospheres, wafers, micelles, liposomes or woven or non-woven fabrics.
1. A drug delivery composition which comprises, based on total weight of the composition:
at least 0.1 wt. % to 99 wt. % of a protein, and from 99.9 wt. % to 0.1 wt. % of a biodegradable polymer, wherein
the biodegradable polymer comprises at least two different functional groups being unprotected hydrophilic groups and protected hydrophobic groups pendant to the polymer backbone, which functional groups are selected from the group of carboxyl, amine, hydroxyl, ester, amide, thiol or thioester groups, and wherein
the unprotected hydrophilic groups and the protected hydrophobic groups are present in a ratio of the unprotected hydrophilic groups to the protected hydrophobic groups of 0.17-3, and wherein
the polymer absorbs between 2-20% w/w water if exposed to physiological conditions for at least 20 days.
2. The drug delivery composition according to claim 1 , wherein the two different functional groups comprise at least an unprotected hydrophilic functional group selected from a carboxyl group, an amine group, a thiol group or a hydroxyl group, and at least a protected hydrophobic functional group selected from an ester group, an amide group or a thioester group.
3. The drug delivery composition according to claim 2 , wherein the biodegradable polymer possesses pendant carboxyl and ester functional groups.
4. The drug delivery composition according to claim 1 , wherein the biodegradable polymer is a polyesteramide.
5. The drug delivery composition according to claim 4 , wherein the polyesteramide is a polyesteramide copolymer according to structural Formula (I):
wherein m+p varies from 0.9-0.1 and a+b varies from 0.1 to 0.9; m+p+a+b=1, wherein m or p could be 0; n varies from 5 to 300 and wherein a is at least 0.01, b is at least 0.015 and the ratio of a to b (a:b) is from 0.1:9 to 0.85:0.15, wherein the m unit and/or p unit, and the a and b units, are randomly distributed; and
wherein:
R 1 is independently selected from the group consisting of (C 2 -C 20 ) alkyl,
R 3 and R 4 in a single backbone unit m or p, respectively, are independently selected from the group consisting of hydrogen, (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 6 -C 10 )aryl, (C 1 -C 6 )alkyl, —(CH 2 )SH, —(CH 2 ) 2 S(CH) 3 ,(CH 3 ) 2 —CH—CH 2 —, —CH(CH 3 ) 2 , —CH(CH 3 )—CH 2 —CH 3 ), —CH 2 —C 6 H 5 , —(CH 2 ) 4 —NH 2 , and mixtures thereof;
R 5 is independently selected from the group consisting of (C 2 -C 20 )alkyl, (C 2 -C 20 )alkylene,
R 6 is selected from bicyclic-fragments of 1,4:3,6-dianhydrohexitols of structural formula (II);
R 7 is independently selected from the group consisting of (C 6 -C 10 ) aryl (C 1 -C 6 ) alkyl or a protecting group; and
—R 8 is —(CH 2 ) 4 —.
6. The drug delivery composition according to claim 5 , wherein b is at least 0.025 and a:b is from 0.1:0.9 to 0.75:0.25.
7. The drug delivery composition according to claim 5 , wherein b is at least 0.05 and a:b is from 0.1:0.9 to 0.5:0.5.
8. The drug delivery composition according to claim 5 , wherein p=0 and m+a+b=1, m=0.75, b is 0.125 and a+b=0.25, wherein R 1 is (CH 2 ) 8 , R 3 is (CH 3 ) 2 —CH—CH 2 —, R 5 is hexyl, R 7 is benzyl, R 8 is —(CH 2 ) 4 —.
9. The drug delivery composition according to claim 5 , wherein m+p+a+b=1, a+b=0.25, p=0.45 and m=0.3, b is 0.0625, wherein R 1 , —(CH 2 ) 8 ; R 3 and R 4 respectively, are (CH 3 ) 2 —CH—CH 2 —, R 5 is selected from the group consisting of (C 2 -C 20 )alkylene, R 7 is benzyl, R 8 is —(CH 2 ) 4 —; R 6 is selected from bicyclic-fragments of 1,4:3,6-dianhydrohexitols of structural formula (II).
10. The drug delivery composition according to claim 5 , wherein m+p+a+b=1, a+b=0.25, p=0.45 and m=0.3, b is 0.125, wherein R 1 is —(CH 2 ) 8 ; R 4 is (CH 3 ) 2 —CH—CH 2 —, R 7 is benzyl, R 8 is —(CH 2 ) 4 ; R 6 is selected from bicyclic-fragments of 1,4:3,6-dianhydrohexitols of structural formula (II).
11. The drug delivery composition according to claim 5 , wherein m+p+a+b=1, a+b=0.1, p=0.30 and m=0.6, b is 0.05, wherein R 1 —(CH 2 ) 4 ; R 3 and R 4 respectively, are (CH 3 ) 2 —CH—CH 2 —; R 7 benzyl, R 8 is —(CH 2 ) 4 —; R 5 is selected from the group consisting of (C 2 -C 20 )alkylene, R 6 is selected from bicyclic-fragments of 1,4:3,6-dianhydrohexitols of structural formula (II).
12. The drug delivery composition according to claim 1 , wherein the protein is at least one selected from the group consisting of peptides, insulin, hormones, vaccines, enzymes, antibiotics, antibodies, neuroactive agents, growth factors, cytokines, antigens and glycoproteins.
13. A drug delivery system for controlled protein release comprising the composition according to claim 1 .
14. The drug delivery system according to claim 13 , wherein the drug delivery system is in a form comprising microparticles, films, coatings, fibers, pellets, cylinders, discs, implants, microcapsules, microspheres, nanospheres, wafers, micelles, liposomes, woven or non-woven fabrics.
15. The drug delivery composition according to claim 2 , wherein the unprotected hydrophilic groups and the protected hydrophobic groups are present in a ratio of the unprotected hydrophilic groups to the protected hydrophobic groups of 0.3-1.
16. The drug delivery composition according to claim 3 , wherein the unprotected hydrophilic groups and the protected hydrophobic groups are present in a ratio of the unprotected hydrophilic groups to the protected hydrophobic groups of 0.3-1.
17. The drug delivery composition according to claim 2 , wherein the composition comprises from 0.1 wt% to 20 wt% of the protein and from 60 wt% to 99.9 wt% of the biodegradable polymer.
18. The drug delivery composition according to claim 3 , wherein the composition comprises from 0.1 wt% to 20 wt% of the protein and from 6 wt% to 99.9 wt% of the biodegradable polymer.
19. The drug delivery composition according to claim 5 , wherein the composition comprises from 0.1 wt% to 20 wt% of the protein and from 60 wt% to 99.9 wt% of the biodegradable polymer.
20. The drug delivery composition according to claim 8 , wherein the composition comprises from 0.1 wt% to 20 wt% of the protein and from 60 wt% to 99.9 wt% of the biodegradable polymer.