HIV-1 GP 120 V1/V2 antigens and immunological uses thereof
HIV-1 envelope proteins and fragments that possess naturally occurring and novel engineered epitopes that can be used to elicit (and are recognized by) broadly neutralizing antibodies.
1. An immunogenic composition comprising an MN-rgp120 polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, and SEQ ID NO: 7.
2. The immunogenic composition of claim 1 , wherein the MN-rgp120 polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 3.
3. The immunogenic composition of claim 1 , wherein the MN-rgp120 polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 4.
4. The immunogenic composition of claim 1 , wherein the MN-rgp120 polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 5.
5. The immunogenic composition of claim 1 , wherein the MN-rgp120 polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 6.
6. The immunogenic composition of claim 1 , wherein the MN-rgp120 polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 7.
7. A method for producing an MN-rgp120 polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, and SEQ ID NO: 7, the method comprising expressing the MN-rgp120 polypeptide in a cell line comprising a nucleic acid encoding the MN-rgp120 polypeptide.
8. The method of claim 7 , wherein the cell line lacks N-acetylglucosaminyltransferase I (GnTI) activity.
9. A method for inducing an immune response to an MN-rgp120 polypeptide in a subject, the method comprising administering the MN-rgp120 polypeptide to the subject, where the MN-rgp120 polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, and SEQ ID NO: 7.
10. The method of claim 9 , wherein the immune response comprises generation of broadly neutralizing antibodies that bind to the MN-rgp120 polypeptide.