Ergothioneine production through metabolic engineering
The present disclosure relates to the production of ergothioneine through either in vitro enzymatic transformations or fermentations using microbials created by metabolic engineering. Also disclosed are transformed cells useful in such methods and ergothioneine produced by such methods. Transformed cells of the disclosure are capable of converting histidine and cysteine or hercynine and cysteine into ergothioneine in greater efficiency than the untransformed wild-type cells.
1. A process for preparing ergothioneine, the process comprising:
(i) incubating histidine or hercynine with a reaction mixture comprising recombinantly expressed:
(a) EgtA protein,
(b) EgtB protein,
(c) EgtC protein,
(d) EgtD protein, and
(e) EgtE protein, wherein the EgtE protein is produced from recombinant coexpression with a gene encoding a FAD synthetase to facilitate EgtE production; and
(ii) isolating ergothioneine from the reaction mixture.
2. A process for preparing ergothioneine, the process comprising:
(i) incubating hercynine with a reaction mixture comprising recombinantly expressed:
(a) OvoA protein and
(b) EgtE protein; and
(ii) isolating ergothioneine from the reaction mixture.
3. A process of claim 2 , wherein the reaction mixture further comprises recombinantly expressed EgtD protein.
4. The process of claim 1 , wherein the EgtD protein is recombinantly co-expressed with a gene encoding SAM synthetase.
5. The process of claim 1 , wherein the enzymatic mixture further comprises methionine or cysteine.
6. The process of claim 1 , wherein the enzymatic mixture further comprises an iron salt.
7. The process of claim 1 , wherein the reaction mixture comprises a cell lysate.
8. A process for preparing ergothioneine, the process comprising:
(i) incubating hercynine with a reaction mixture comprising recombinantly expressed:
(a) NcEgt1 protein and
(b) EgtE protein, wherein the EgtE protein is produced from recombinant coexpression with a gene encoding a FAD synthetase to facilitate EgtE production; and
(ii) isolating ergothioneine from the reaction mixture.
9. A process of claim 8 , wherein the reaction mixture further comprises recombinantly expressed EgtD protein.