Compositions and methods conferring resistance of maize to corn rootworm II
Methods and compositions for increasing a plant's resistance to an insect pest such as the corn rootworm are provided. Methods are provided for overexpression of Crw2, or variants thereof, in a host plant or plant cell to increase resistance to an insect pest in a plant such as maize. Methods are also provided for identifying variants of Crw2 that when incorporated into a plant via transgenic or traditional breeding means increase resistance to an insect pest in a plant such as maize. Also provided are methods for increasing resistance by overexpressing Crw1 and Crw2.
1. A method of increasing resistance to herbivory by corn rootworm in a plant, said method comprising:
(a) introducing into a regenerable plant cell a recombinant DNA construct comprising a polynucleotide operably linked to at least one regulatory sequence, wherein the polynucleotide encodes a polypeptide having an amino acid sequence of at least 95% sequence identity when compared to SEQ ID NO:2; and wherein the at least one regulatory sequence is a root-specific promoter, and
(b) regenerating a transgenic plant from the regenerable plant cell after step (a), wherein the transgenic plant comprises in its genome the recombinant DNA construct; and
wherein the transgenic plant has increased resistance to corn rootworm when compared to a control plant not comprising the recombinant DNA construct.
2. The method of claim 1 , further comprising:
(d) obtaining a progeny plant derived from the transgenic plant, wherein said progeny plant comprises in its genome the recombinant DNA construct and exhibits increased resistance to herbivory by corn rootworm when compared to a control plant not comprising the recombinant DNA construct.
3. The method of claim 1 , wherein said plant is a monocot.
4. The method of claim 3 , wherein said monocot is maize.