Flavivirus associated with Theiler's disease
View Patent ↗This disclosure relates to Theiler's disease-associated virus (“TDAV”), reagents relating thereto, and methods for detecting, using, and/or treating diseases associated with TDAV.
1. A primer pair selected from the group consisting of SEQ ID NOS.: 4 and 5; SEQ ID NOS.: 6 and 7; SEQ ID NOS.: 8 and 9; SEQ ID NOS.: 10 and 11; SEQ ID NOS.: 12 and 13; SEQ ID NOS.: 14 and 15; SEQ ID NOS.: 16 and 17; and SEQ ID NOS.: 18 and 19;
wherein at least one primer of the pair comprises a detectable label.
2. A method for detecting a virus in a sample, comprising amplifying from the sample a nucleic acid using the primer pair of claim 1 .
3. The method of claim 2 further comprising detecting the amplified nucleic acid.
4. A kit for the detection of nucleic acid of a virus in a sample, the kit comprising the primer pair of claim 1 ; and one or more amplification reagents.
5. The kit of claim 4 wherein the primer pair is fixably attached to a solid support.
6. The kit of claim 4 further comprising a labeled oligonucleotide probe for detecting the amplified nucleic acid.
7. A solid support comprising fixably bound thereto at least one primer of the primer pair of claim 1 .
8. A method for detecting Theiler's disease-associated virus (TDAV) in a sample, comprising amplifying from the sample a nucleic acid using a primer pair selected from the group consisting of SEQ ID NOS.: 4 and 5; SEQ ID NOS.: 6 and 7; SEQ ID NOS.: 8 and 9; SEQ ID NOS.: 10 and 11; SEQ ID NOS.: 12 and 13; SEQ ID NOS.: 14 and 15; SEQ ID NOS.: 16 and 17; and SEQ ID NOS.: 18 and 19.