QUANTITATIVE LATERAL FLOW ASSAY

1 . A lateral flow test device for quantitatively detecting multiple analytes in a sample, which device comprises a porous matrix that comprises at least two distinct test locations on said porous matrix, each of said test locations comprising a test reagent that binds to an analyte or another binding reagent that binds to said analyte, or is an analyte or an analyte analog that competes with an analyte in said sample for binding to a binding reagent for said analyte, and said test reagents at said at least two test locations bind to at least two different analytes or different binding reagents that bind to said different analytes, or are different analytes or analyte analogs, wherein a liquid sample flows laterally along said test device and passes said test locations to form a detectable signal to determine amounts of said multiple analytes in said sample.

2 . A method for quantitatively detecting multiple analytes in a sample, which method comprises:

a) contacting a liquid sample with the test device of claim 1 , wherein the liquid sample is applied to a site of the test device upstream of the test locations;

b) transporting multiple analytes, if present in the liquid sample, and a labeled reagent to the test locations; and

c) assessing a detectable signal at the test locations to determine the amounts of the multiple analytes in the sample, wherein the amount of each of the analytes is determined.

3 . A system for quantitatively detecting multiple analytes in a sample, which system comprises:

a) a test device of claim 1 ; and

b) a reader that comprises a light source and a photodetector to detect a detectable signal.

4 . A kit for quantitatively detecting multiple analytes in a sample, which kit comprises:

a) a test device of claim 1 ; and

b) an instruction for using the test device to quantitatively detect multiple analytes in a sample.