Technique for laser-cutting an endothelial corneal graft

1. A method of preparing an endothelial corneal graft using laser radiation, the method comprising:

providing a donor cornea isolated from a dead body by inverting a natural curvature of the donor cornea, wherein a natural shape is that of a bowl with an epithelium layer on an outer side of the bowl and an endothelium layer on the inner side, and an inverted shape has the epithelium layer on the inner side and the endothelium layer on the outer side;

clamping the donor cornea with an inverted shape onto a socket, forming an artificial interior chamber between the endothelium layer and the socket, the socket further including a fluid line;

arranging the socket such that the donor cornea is positioned between the socket and a contact surface contact surface of a cutting laser interface of a laser apparatus that generates the laser radiation;

pressuring, using the fluid line, the artificial interior chamber to cause the endothelium layer to abut the contact surface of the cutting laser interface;

irradiating the donor cornea from the endothelial side thereof with laser radiation to cause a photodisruption in tissue of the donor cornea at a focal point of the radiation; and

moving the focal point of the radiation to form an endothelial graft in the donor cornea.

2. The method of claim 1 , comprising:

determining a thickness of a predetermined structure of the donor cornea, the predetermined structure formed by one of an endothelium, a Descemet membrane and a Dua layer of the donor cornea or a combination thereof;

based on the determined thickness of the predetermined structure, determining a desired thickness of the endothelial graft;

wherein moving the focal point of the radiation includes moving the focal point across a surface that is a distance corresponding to the desired thickness of the endothelial graft from the reference contact surface.

3. The method of claim 2 , wherein the desired thickness of the endothelial graft is determined to be in a range from 30 to 120 μm.

4. The method of claim 2 , wherein the surface extends at least substantially parallel to the reference contact surface.

5. The method of claim 1 , wherein providing the donor cornea includes positioning the donor cornea on a support unit in opposition to the contact surface of a laser apparatus generating the laser radiation.

6. The method of claim 1 , wherein irradiating the donor cornea includes focusing the laser radiation in stromal tissue of the donor cornea, wherein moving the focal point of the radiation includes moving the focal point through the stromal tissue to create a stromal bed cut for the graft.

7. The method of claim 6 , wherein at least a major part of the bed cut extends substantially parallel to a Descemet membrane of the donor cornea.

8. The method of claim 6 , comprising: selecting a desired distance between the bed cut and a Descemet membrane in the range from 5 μm to 100 μm, wherein moving the focal point of the radiation includes moving the focal point through the stromal tissue at the selected distance from the Descemet membrane.

9. The method of claim 8 , wherein selecting a desired distance includes selecting the desired distance differently for different wavelengths of the radiation.

10. The method of claim 1 , wherein moving the focal point of the radiation includes moving the focal point so as to leave undisrupted a connection structure connecting the endothelial graft with surrounding tissue of the donor cornea.

11. The method of claim 10 , wherein the connection structure is formed by stromal tissue of the donor cornea.

12. The method of claim 5 , further comprising:

enclosing the donor cornea in a volume between the support unit and the contact surface of the laser apparatus.

13. The method of claim 12 , further comprising:

creating a vacuum in the enclosed volume between the support unit and the contact surface of the laser apparatus to further abut the endothelium and a contact surface of a laser apparatus.