The invention provides proteins from Neisseria meningitidis , including the amino acid sequences and the corresponding nucleotide sequences. The proteins are predicted to be useful antigens for vaccines and/or diagnostics.
1. A method of preparing an immunogenic composition comprising:
(a) expressing in E. coli a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO: 2536, wherein the fragment comprises 16 or more consecutive amino acids from the amino acid sequence, wherein the polypeptide is immunogenic;
(b) purifying the polypeptide;
(c) preparing the immunogenic composition by combining the purified polypeptide with an aluminum salt adjuvant.
2. The method of claim 1 , wherein the fragment comprises 20 or more consecutive amino acids from the amino acid sequence of SEQ ID NO: 2536.
3. The method of claim 1 , wherein the immunogenic composition further comprises a pharmaceutically acceptable carrier.
4. The method of claim 1 , wherein the aluminum salt is aluminum phosphate.
5. The method of claim 3 , wherein the aluminum salt is aluminum phosphate.
6. The method of claim 1 , wherein the immunogenic composition further comprises a pH buffering agent.
7. The method of claim 6 , wherein the immunogenic composition further comprises a pH buffering agent.
8. A method of preparing an immunogenic composition comprising combining a purified polypeptide with an aluminum salt adjuvant, wherein the purified polypeptide was expressed in, and purified from, E. coli ; comprises a fragment of the amino acid sequence of SEQ ID NO: 2536, wherein the fragment comprises 16 or more consecutive amino acids from the amino acid sequence; and is immunogenic.
9. The method of claim 8 , wherein the fragment comprises 20 or more consecutive amino acids from the amino acid sequence of SEQ ID NO: 2536.
10. The method of claim 8 , wherein the immunogenic composition further comprises a pharmaceutically acceptable carrier.
11. The method of claim 8 , wherein the aluminum salt is aluminum phosphate.
12. The method of claim 10 , wherein the aluminum salt is aluminum phosphate.
13. The method of claim 8 , wherein the immunogenic composition further comprises a pH buffering agent.
14. The method of claim 12 , wherein the immunogenic composition further comprises a pH buffering agent.