DNA amplification and sequencing using DNA molecules generated by random fragmentation
The present invention is directed to methods to prepare a DNA molecule or a plurality of DNA molecules by random fragmentation. In some embodiments, the present invention regards preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3′ end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, a library is prepared with methods of the present invention.
1. An in vitro method of incorporating a 3′ OH group onto the 3′ end of a nucleic acid molecule having a 3′ end that lacks an OH group, the method comprising the steps of:
a) obtaining a nucleic acid molecule having a 3′ end that lacks a 3′ OH group; and
b) incorporating a 3′ OH group onto the 3′ end that lacks an OH group by exposing the molecule to a terminal deoxynucleotidyltransferase in the presence of a guanine ribonucleotide, guanine deoxyribonucleotide, or both, thereby generating a conditioned nucleic acid molecule.
2. The method of claim 1 , wherein a guanine homopolymer extension is generated on the non-extendable 3′ end.
3. The method of claim 1 , further comprising generating at least one blunt end on the conditioned nucleic acid molecule.
4. The method of claim 1 , further comprising ligating an adaptor molecule to at least one end of the conditioned nucleic acid molecule.
5. The method of claim 1 , further comprising amplifying the conditioned nucleic acid molecule.
6. The method of claim 1 , wherein the 3′ end that lacks an OH group is the result of heat-induced damage.
7. The method of claim 1 , wherein the 3′ end that lacks an OH group comprises a dideoxy terminus.
8. The method of claim 1 , wherein the 3′ end that lacks an OH group comprises an amino-blocked terminus.
9. The method of claim 1 , wherein the nucleic acid molecule having a 3′ end that lacks a 3′ OH group is a double-stranded DNA.
10. The method of claim 9 , wherein the 3′ end that lacks an OH group is at a nick in the double-stranded DNA.
11. The method of claim 1 , wherein the nucleic acid molecule having a 3′ end that lacks a 3′ OH group is a product of random fragmentation.
12. The method of claim 11 , wherein the random fragmentation comprises mechanical fragmentation, chemical fragmentation, or enzymatic fragmentation.
13. The method of claim 12 , wherein the mechanical fragmentation comprises hydrodynamic shearing, sonication, or nebulization.
14. The method of claim 12 , wherein the chemical fragmentation comprises acid catalytic hydrolysis, alkaline catalytic hydrolysis, hydrolysis by metal ions, hydroxyl radicals, irradiation, or heating.
15. The method of claim 12 , wherein the enzymatic fragmentation comprises digestion with DNase I.
16. The method of claim 1 , wherein the guanine ribonucleotide, guanine deoxynucleotide, or both are labeled.
17. The method of claim 16 , wherein the label comprises a radionuclide, an affinity tag, a hapten, an enzyme, a chromophore, or a fluorophore.