Immunoassay for phenethylamines of the 2C and DO sub-families
View Patent ↗Immunoassay methods and their requisite components for the detection and determination of phenethylamines of the 2C and DO sub-families are described.
1. A polyclonal antibody which specifically binds to 1-(4-bromo-2,5-dimethoxyphenyl)propan-2-amine (DOB), 1-(2,5-dimethoxy-4-methylphenyl)propan-2 -amine (DOM), and 1-(2,5-dimethoxy-4-nitrophenyl)propan-2-amine (DON), wherein the polyclonal antibody shows a cross-reactivity of less than 1.0% for each of dimethylamylamine, methamphetamine, amphetamine, (+)-phenethylamine, 1-(4 -bromo-2,5-dimethoxyphenyl)ethan-2-amine (2C-B), 1-(2,5-di methoxy-4-propylphenyl)ethan-2-amine (2C-P), 1-(2,5-di methoxy-4-ethylphenyl)ethan-2-amine (2C-E), 1-(2,5-dimethoxy-4-ethylthiophenyl)ethan-2-amine (2C-T2), 1-(4-chloro-2,5 -dimethoxyphenyl)ethan-2-amine (2C-C), 1-(2,5-dimethoxy-4-propylthiophenyl)ethan-2 -amine (2C-T-7), 1-(2,5-dimethoxy4-methylphenyl)ethan-2-amine (2C-D), and 1-(4-cyano-2,5-dimethoxyphenyl)ethan-2-amine (2C-CN), when compared to 100% cross-reactivity for DOB, wherein said cross reactivity is measured in the presence of a conjugate of structure:
2. The polyclonal antibody of claim 1 , wherein said antibody is raised against an immunogen of structure:
3. The polyclonal antibody of claim 1 , wherein the antibody shows a sensitivity (IC 50 ) of less than 2.5 ng/mL for DOB.
4. An immunoassay method of detecting phenethylamines of DO sub-family, the method comprising contacting a sample, wherein the sample is suspected of containing the phenethylamines of the DO sub-family, with one or more detecting agents and the polyclonal antibody of claim 1 ; measuring a signal or signals produced by the one or more detecting agents; and deducing from a calibration curve the presence of, or amount of, the phenethylamines of the DO sub-family.