STEM CELL BASED ANTI-CANCER COMPOSITIONS AND METHODS
The present invention provides compositions and methods for target radiotherapeutic treatment of cancer. Freshly isolated or culture expanded ELA stem cells are loaded with at least one of a diagnostic agent and a therapeutic agent, and the stem cells are then introduced into a subject. The loaded ELA stem cells migrate to location of cancer and form the basis both of diagnostic imaging and of directed Neutron Capture Therapy. Treatment of a subject may include ELA stem cell-based NCT radiotherapy as an adjunct to traditional therapies. An ELA stem cell-based diagnostic preparation can be used directly, or as a further addition to current imaging agents and methods.
1 . A cell-based preparation comprising: a population of loaded ELA stem cells further comprising a diagnostic agent and a therapeutic agent.
2 . The cell-based preparation of claim 1 , wherein the diagnostic agent is Gadolinium-157.
3 . The cell-based preparation of claim 1 , wherein the therapeutic agent is selected from at least one of Gadolinium-157 and Boron-10.
4 . The cell-based preparation of claim 1 , wherein the loaded ELA stem cells are in an amount of 10 2 to 10 8 .
5 . The cell-based preparation of claim 4 , wherein the loaded ELA stem cells are in a volume of 0.5 to 10 ml.
6 . The cell-based preparation of claim 4 , further comprising a pharmaceutically acceptable solution.
7 . The cell-based preparation of claim 1 , further comprising a perfluorate agent.
8 . The cell-based preparation of claim 4 , further comprising unloaded ELA stem cells, wherein the ratio of the loaded cells to the unloaded cells is greater than 2:1.
9 . The cell-based preparation of claim 8 , wherein the ratio of the loaded cells to the unloaded cells is greater than 5:1.
10 . A method of treating a subject having a cancer or a neoplasm, the method comprising:
introducing to the subject a quantity of the cell-based preparation of claim 1 or claim 4 which targets to the neoplasm, and
administering radiotherapy by exposing the subject to a neutron source of greater than 0.5 eV to about 10 keV, thereby treating the neoplasm of the subject by the radiotherapy.
11 . The method according to claim 10 , further comprising prior to exposing, imaging the subject.
12 . The method of claim 10 , further comprising prior to introducing, obtaining from the subject a population of ELA cells, isolating the ELA cells and extending the ELA cells by culture prior to loading and administration to the subject.
13 . A patient-specific master cell bank prepared by the method of claim 12 prior to introducing to the subject the cell-based preparation.
14 . A kit comprising: a single dose cell-based preparation of loaded ELA stem cells of claim 1 , appropriate sterile packaging, and instructions for use.
15 . A method of manufacture for a cell-based preparation comprising: obtaining a population of ELA stem cells, and culturing the cells under conditions suitable to permit uptake by the cells of a diagnostic agent and a therapeutic agent.
16 . The method of claim 15 , wherein the diagnostic and therapeutic agents is selected from at least one of Gadolinium-157 and Boron-10.
17 . A cell bank of ELA cell lines each specific to a patient, wherein populations of the ELA cells are obtained from the patient, and are isolated and are expanded by culture to obtain a patient-specific ELA cell bank.