IP Library Granted Patent US 9,322,012
Granted Patent B2
US 9,322,012 · App. 14/455,780 · Granted Apr 26, 2016

Enzyme compositions

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Quick Facts
Patent No.
US 9,322,012
App. No.
14/455,780
Granted
Apr 26, 2016
Kind
B2
Abstract

The present technology provides for enzyme compositions with enhanced enzyme activity, thermophilic and psychrophilic stability.

Claims (13)

1. An enzyme composition comprising:

one or more self-assembled lipid-functionalized graphene or lipid-functionalized fullerene nano-cages; and

at least one enzyme entrapped by, but not linked to, the lipid-functionalized graphene or the lipid-functionalized fullerene nano-cage, wherein the entrapped enzyme in the composition has an enhanced activity as compared to an untrapped enzyme.

2. The composition of claim 1 , wherein the entrapped enzyme comprises one or more of a laccase, a pectinase, a protease, a cellulase and a xylanase.

3. The composition of claim 1 , wherein the entrapped enzyme comprises one or more of psychrophilic enzymes, mesophilic enzymes, and thermophilic enzymes.

4. The composition of claim 1 , further comprising a plurality of nanoparticles, wherein the nanoparticles are in contact with, but not linked to, the enzyme.

5. The composition of claim 4 , wherein the nanoparticles comprise one or more of hydroxyapatite, copper, magnesium chloride, manganese chloride, calcium chloride, zinc, magnesium, and manganese.

6. The composition of claim 1 , wherein the lipid-functionalized graphene or lipid-functionalized fullerene nano-cages, comprise at least one lipid comprising one or more of a phospholipid, a sphingolipid, a phosphosphingolipid, and a steroid.

7. The composition of claim 1 , wherein the lipid-functionalized fullerene comprises at least one lipid-functionalized carbon nanotube.

8. The composition of claim 7 , wherein the carbon nanotube comprises one or more of single-wall carbon nanotubes (SWCNT), double-walled carbon nanotubes, or multi-walled carbon nanotubes.

9. The composition of claim 7 , wherein the ratio (wt/wt) of lipid-functionalized carbon nanotubes to the enzyme is about 6:1 to about 2:1.

10. The composition of claim 7 , wherein the ratio (wt/wt) of lipids to carbon nanotubes is about 5:1.

11. The composition of claim 1 , wherein the entrapped enzyme comprises a recombinant enzyme.

Assignments (3)
RELEASE OF SECURITY INTEREST Recorded Jul 31, 2019
From: CRESTLINE DIRECT FINANCE, L.P.
To: EMPIRE TECHNOLOGY DEVELOPMENT LLC
Reel/Frame 049924/0794 →
SECURITY INTEREST Recorded Jan 29, 2019
From: EMPIRE TECHNOLOGY DEVELOPMENT LLC
To: CRESTLINE DIRECT FINANCE, L.P.
Reel/Frame 048373/0217 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 1, 2016
From: DASGUPTA, ANJAN KR.; BHATTACHARYYA, TAMOGHNA; MUKHOPADHYAY, ARKA; DUTTA, NALOK; CHAKRABORTY, KRISHANU
To: UNIVERSITY OF CALCUTTA
Reel/Frame 037630/0069 →