System and process for separating a material
Disclosed is a system to separate, enrich, and/or purify a cellular population from a biological tissue, such as a tissue sample. For example, an adipose tissue sample can be acquired and disrupted. The disrupted tissue sample can then be separated and purified. The separated components can include multipotent, pluripotent, or other cell populations.
1. A system to separate a selected cell fraction from a sample volume, comprising:
a container having a first wall extending from a first bottom wall to a an upper rim having an interior surface and an exterior surface;
a collection section positioned near the upper rim and at least contacting the exterior surface of the first wall, the collection section having a second wall extending from a second bottom wall to the upper rim, and wherein the second bottom wall is nearer the upper rim than the first bottom wall;
a lid assembly extending over both the container and the collection section;
a sealing section operable to seal a collection volume within the collection section from a container volume in the container;
a flexible section operable to allow the sealing section to move between an open position and a closed position; and
a drive motor operable to rotate the container and the collection section simultaneously;
wherein in the closed position the sealing section selectively seals the collection volume from the container volume;
wherein at a selected spin rate the sealing section moves to the open position from the closed position.
2. The system of claim 1 , further comprising:
a sealing member positioned in an annular depression surrounding the container and positioned at the upper rim;
wherein the sealing section contacts the sealing member to seal collection volume from the container volume.
3. The system of claim 2 , wherein the disruption system includes a perforated member defining at least a through bore;
a sample containing volume having an end covered by the perforated member; and
a forcing member operable to force the sample volume through the perforated member to achieve a sample particle.
4. The system of claim 1 , further comprising:
a disruption system operable to form a sample particle from a sample volume.
5. The system of claim 4 , wherein the disruption system is operable to form a sample particle having an average particle size in a range of about 0.1 mm to about 5 mm.
6. The system of claim 4 , wherein the disruption system is operable to form a sample particle having an average particle size in a range of about 0.5 mm to about 2 mm.
7. The system of claim 4 , wherein the disruption system is operable to form a sample particle having an average particle size of about 1 mm.
8. The system of claim 1 , further comprising:
a housing operable to enclose all of the container, the collection section, the lid, the sealing section, the flexible section, and the drive motor.
9. The system of claim 1 , further comprising:
an outer housing operable to contain the container, the collection section, the lid, the sealing section, and the flexible section; and
a drive base operable to contain the drive motor;
wherein the outer housing is removeably attachable to the drive base.
10. The system of claim 1 , wherein the collection section defines an annular well surrounding the container;
a barrier wall defining a first end and a second end of a collection section, wherein the barrier wall disrupts the complete annular well of the collection section;
wherein a vacuum is operable to be formed near the first end of the collection section to draw substantially all material out of the collection section through a port.
11. The system of claim 1 , wherein the flexible section is formed by a portion of the lid assembly and a portion of the flexible section forms the sealing section, wherein the flexible section is configured to flex to provide an opening between the collection volume and the container volume.
12. The system of claim 1 , where the lid assembly includes a first flexible lid forming the flexible section and sealing section and a second lid positioned atop the first flexible lid.
13. A system to separate a selected cell fraction from a sample volume, comprising:
a container having a main container area and an isolation area spaced from the main container area with a wall portion therebetween; and
a lid covering both the main container area and the isolation area, the lid including,
a sealing portion operable to seal the main container area from the isolation area, and
a flexible portion operable to allow the sealing portion to move between an open position and a closed position,
wherein in the closed position the sealing position seals the main container area from the isolation area and in the open position the isolation area is opened to the main container area.
14. The system of claim 13 , further comprising an outer housing, wherein the container having the main container area and the isolation area are contained within the outer housing.
15. The system of claim 14 , further comprising a drive base having a drive motor, the drive base being coupled to the outer housing and the container to rotate the container about a central axis.
16. The system of claim 13 , wherein the isolation area is an annular well surrounding the main container area, where the isolation area includes a barrier wall extending a width of the isolation area, where the barrier wall defines a first end and a second end of the isolation area.
17. The system of claim 13 , wherein a portion of the flexible portion of the lid forms the sealing portion.
18. The system of claim 17 , further comprising an outer covering lid positioned atop the lid, wherein the outer covering lid provides a limit to the amount of flexing of the lid.
19. A system to separate a selected cell fraction from a sample volume, comprising:
a container having a main container area and an isolation area spaced from the main container area with a wall portion therebetween, the isolation area forming an annular well around the main container area;
a barrier wall extending a width of the isolation area, where the barrier wall defines a first end and a second end of the isolation area; and
a lid covering both the main container area and the isolation area, the lid including,
a sealing portion operable to seal the main container area from the isolation area, and
a flexible portion operable to allow the sealing portion to move between an open position and a closed position,
wherein in the closed position the sealing portion seals the main container area from the isolation area and in the open position the main container area is open to the isolation area.
20. The system of claim 19 , further comprising an outer covered lid positioned atop the lid, wherein the outer covered lid provides a limit to the amount of flexing of the lid.
21. The system of claim 19 , wherein the annular well includes a sump defined within the annular well and a port positioned relative to the sump, wherein the selected cell fraction is operable to collect within the sump defined within the isolation area and a suction force can be applied at the first end to move the selected cell fraction within the isolation area.
22. The system of claim 19 , further comprising a drive motor operable to rotate the container about a central axis, wherein at a selected spin rate the lid is operable to flex at a periphery with the flexible portion to move the sealing portion from the closed position to the open position.