Antibodies that specifically bind to TIM3
Provided herein are antibodies specific for TIM3 that can be used to detect cancer cells, in particular, cancer stem cells. The antibodies can also be used in therapeutic compositions for treating cancer and reducing inflammation.
1. An antibody that specifically binds the extracellular domain of human T-cell Immunoglobulin Mucin (TIM) 3, wherein said binding results in internalization into a TIM3-expressing cell, and wherein the antibody is selected from the group consisting of
(i) an antibody having light chain complementarity determining region (CDR) sequences of SEQ ID NOs:8-10 and heavy chain CDR sequences of SEQ ID NOs:3-5;
(ii) an antibody having light chain CDR sequences of SEQ ID NOs:18-20 and heavy chain CDR sequences of SEQ ID NOs:13-15;
(iii) an antibody having light chain CDR sequences of SEQ ID NOs:28-30 and heavy chain CDR sequences of SEQ ID NOs:23-25;
(iv) an antibody having light chain CDR sequences of SEQ ID NOs:108-110 and heavy chain CDR sequences of SEQ ID NOs:103-105; and
(v) an antibody having light chain CDR sequences of SEQ ID NOs:118-120 and heavy chain CDR sequences of SEQ ID NOs:113-115.
2. The antibody of claim 1 , wherein said TIM3 -expressing cells are acute myeloid leukemia (AML) cells.
3. The antibody of claim 1 , wherein the TIM3 -expressing cells are B lymphoma cells.
4. The antibody of claim 1 , wherein the antibody has light chain complementarity determining region (CDR) sequences of SEQ ID NOs:8-10 and heavy chain CDR sequences of SEQ ID NOs:3-5.
5. The antibody of claim 1 , wherein the antibody has light chain CDR sequences of SEQ ID NOs:18-20 and heavy chain CDR sequences of SEQ ID NOs:13-15.
6. The antibody of claim 1 , wherein the antibody has light chain CDR sequences of SEQ ID NOs:28-30 and heavy chain CDR sequences of SEQ ID NOs:23-25.
7. The antibody of claim 1 , wherein the antibody has light chain CDR sequences of SEQ ID NOs:108-110 and heavy chain CDR sequences of SEQ ID NOs:103-105.
8. The antibody of claim 1 , wherein the antibody has light chain CDR sequences of SEQ ID NOs:118-120 and heavy chain CDR sequences of SEQ ID NOs: 113-115.
9. The antibody of claim 1 , wherein said antibody is cross-linked or multimerized.
10. The antibody of claim 1 , wherein the antibody is a chimeric antibody.
11. The antibody of claim 1 , wherein the antibody is a humanized antibody.
12. A pharmaceutical composition comprising the antibody of claim 1 .