Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
1. A recombinant vector or isolated host cell comprising recombinant nucleic acids encoding a protein having at least 90% identity to SEQ ID NO: 77, wherein the protein has lysophosphatidic acid acyltransferase (LPAAT) activity.
2. A recombinant vector or isolated host cell comprising recombinant nucleic acids encoding a protein having at least 90% identity to SEQ ID NO: 78, wherein the protein has lysophosphatidic acid acyltransferase (LPAAT) activity.
3. A recombinant vector or isolated host cell comprising recombinant nucleic acids encoding a protein having at least 90% identity to SEQ ID NO: 79, wherein the protein has lysophosphatidic acid acyltransferase (LPAAT) activity.