Recombinant microorganisms and methods of use thereof
View Patent ↗The present provides selection markers, methods, nucleic acids, and vectors of use in the preparation of recombinant Clostridium spp.
1. A modified phenylalanine tRNA synthetase (PheS) that aminoacylates tRNA with a phenylalanine analogue, wherein the modified PheS has the sequence of SEQ ID NO: 21, except with glycine instead of alanine at amino acid position 311.
2. The modified PheS of claim 1 , wherein the phenylalanine analogue is selected from the group consisting of chlorophenylalanine, fluorophenylalanine and bromophenylalanine.
3. The modified PheS of claim 2 , wherein the phenylalanine analogue is selected from the group consisting of DL-4-chlorophenylalanine, p-chlorophenylalanine, p-fluoro-L-phenylalanine, p-fluoro-DL-phenylalanine, and p-bromo-L-phenylalanine.