Critical point drying of hydrogels in analyte sensors
View Patent ↗A hydrogel is dried by a critical point drying technique. The hydrogel may include indicator molecules embedded therein and may be part of a hydrogel-based device, such as, for example, an analyte sensor.
1. A method of preparing a hydrogel for a hydrogel-based analyte sensor configured for sensing the presence of an analyte in contact with the analyte sensor, the method comprising:
applying the hydrogel to all or part of a surface of a sensor housing of the analyte sensor, wherein the hydrogel comprises indicator molecules that are configured to emit a detectable signal or a change in a detectable signal indicating the presence of the analyte;
applying a protective catalyst to the hydrogel, wherein applying the protective catalyst to the hydrogel comprises sputtering the protective catalyst on the hydrogel; and
drying the hydrogel, which (i) comprises the indicator molecules, (ii) has been applied to all or part of the surface of the sensor housing, and (iii) to which the protective catalyst has been applied, by a critical point drying technique that comprises:
replacing water within the hydrogel with an intermediate fluid that is miscible with water and CO 2 ;
replacing the intermediate fluid within the hydrogel with CO 2 ;
manipulating temperature and pressure to achieve super critical CO 2 ; and
removing the supercritical CO 2 from the hydrogel.
2. The method of claim 1 , wherein the temperature for achieving super critical CO 2 is about 30-35° C., and the pressure for achieving supercritical CO 2 is about 1000-1200 psi.
3. The method of claim 1 , further comprising storing the hydrogel-based analyte sensor at room temperature after the critical point drying step.
4. The method of claim 1 , further comprising implanting the critical point dried hydrogel-based analyte sensor into a living being without pre-hydrating the hydrogel-based analyte sensor prior to implanting.
5. The method of claim 1 , wherein the protective catalyst is platinum.