METHODS AND COMPOSITIONS FOR DETECTING GENETIC MATERIAL
This invention provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some cases, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
1 . A method comprising,
providing target DNA molecules within a plurality of reaction volumes; and
fragmenting the target DNA molecules within the reaction volumes.
2 . The method of claim 1 , wherein the provided target DNA molecules are target DNA fragments.
3 . The method of claim 2 , wherein the target DNA fragments are chromosomal fragments.
4 . The method of claim 2 , wherein the providing comprises fragmenting a DNA sample to generate the target DNA fragments.
5 . The method of claim 1 , wherein said fragmenting comprising contacting the provided target DNA molecules in the reaction volumes with an enzyme that cleaves DNA.
6 . The method of claim 5 , wherein the enzyme is a restriction enzyme.
7 . The method of claim 1 , wherein the reaction volumes are droplets.
8 . The method of claim 7 , wherein the droplets are contained within an emulsion.
9 . The method of claim 1 , wherein the reaction volumes are microcapsules.
10 . The method of claim 2 , wherein the target DNA fragments are less than 10 kilobases (kb) in length.
11 . The method of claim 2 , wherein the providing comprises fragmenting the target DNA molecules outside the reaction volumes to form the target DNA fragments.
12 . The method of claim 1 , wherein the reaction volumes are less than 1 μL, or less than 100 nL in volume.
13 . The method of claim 1 , wherein the m plurality of reaction volumes comprise at least 1,000 or at least 10,000 reaction volumes.