Genetic markers associated with drought tolerance in maize
View Patent ↗The presently disclosed subject matter relates to methods and compositions for identifying, selecting, and/or producing drought tolerant maize plants or germplasm. Maize plants or germplasm that have been identified, selected, and/or produced by any of the methods of the presently disclosed subject matter are also provided.
1. A hybrid Zea mays plant having introgressed into its genome a ZmDr4 water optimization locus that corresponds to SEQ ID NO: 67 [derived from maize variety CML322] that is associated with increased yield at standard moisture percentage (YGSMN) and further wherein the water optimization locus comprises an AA dinucleotide at the position that corresponds to positions 4979-4981 of SEQ ID NO: 7.
2. The Zea mays plant of claim 1 , wherein said introgression of the water optimization locus confers increased or stabilized yield in a water stressed environment as compared to a control plant.
3. The Zea mays plant of claim 1 , wherein the plant is a elite Zea mays plant.
4. The Zea mays plant of claim 1 , wherein the water optimization locus further comprises at least one allele selected from the group consisting of: a deletion at positions 4497-4498 of SEQ ID NO: 7, a G nucleotide at the position that corresponds to position 4505 of SEQ ID NO: 7, a T nucleotide at the position that corresponds to position 4609 of SEQ ID NO: 7, an A nucleotide at the position that corresponds to position 4641 of SEQ ID NO: 7, a T nucleotide at the position that corresponds to position 4792 of SEQ ID NO: 7, a T nucleotide at the position that corresponds to position 4836 of SEQ ID NO: 7, a C nucleotide at the position that corresponds to position 4844 of SEQ ID NO: 7, and a G nucleotide at the position that corresponds to position 4969 of SEQ ID NO: 7.
5. The Zea mays plant of claim 1 , wherein the said plant further comprises in its genome any one of the following haplotypes selected from the group consisting of: haplotype A, haplotype C, haplotype D, haplotype E, haplotype F, haplotype G, haplotype H, haplotype I, haplotype J, haplotype K, haplotype L, and haplotype M.
6. The Zea mays plant of claim 1 , wherein the plant is a Stiff Stalk maize variety or a non-Stiff Stalk maize variety.
7. The Zea mays plant of claim 6 , wherein the plant is any one of NP2391, NP2460 or a progeny of NP2391 or NP2460.
8. The Zea mays plant of claim 1 , wherein the ZmDr4 locus comprises SEQ ID NO: 7.
9. The Zea mays plant of claim 3 , wherein the plant further comprises a transgene that encodes a gene product that provides resistance to a herbicide selected from among glyphosate, Sulfonylurea, imidazolinione, dicambia, glufisinate, phenoxy proprionic acid, cycloshexome, traizine, benzonitrile, and broxynil.
10. A plant cell, plant seed or plant part derived from the plant of claim 1 ;
wherein the plant cell, plant seed or plant part comprises said ZmDr4 water optimization locus.
11. A progeny plant derived from the plant of claim 1 ;
wherein the progeny plant comprises said ZmDr4 water optimization locus.