METHODS OF MAKING SURFACTANT AND CLEANING COMPOSITIONS THROUGH MICROBIALLY PRODUCED BRANCHED FATTY ALCOHOLS
The invention provides a surfactant and/or a cleaning composition comprising a microbially produced branched fatty alcohol or a derivative thereof. The invention also provides a household cleaning composition and a personal or pet care cleaning composition comprising a microbially produced branched fatty alcohol or a derivative thereof.
1 - 31 . (canceled)
32 : A method of making a surfactant composition using branched chain fatty alcohols produced in a recombinant host cell, the method comprising,
(a) providing a recombinant host cell genetically modified to comprise
(i) a polynucleotide encoding a polypeptide comprising one or more subunits having branched chain alpha-keto acid dehydrogenase (BKD) activity (E.C. 1.2.4.4.) capable of catalyzing a conversion of an alpha-keto acid to a branched acyl-CoA,
(ii) a polynucleotide encoding a polypeptide having beta-ketoacyl-ACP synthase (FabH) activity capable of catalyzing a condensation of a branched acyl-CoA and a malonyl-ACP to produce a branched acyl-ACP, and
(iii) a polynucleotide encoding a polypeptide having fatty aldehyde biosynthesis activity capable of catalyzing a conversion of a branched fatty acyl-ACP into a branched fatty aldehyde;
(b) culturing the recombinant host cell in the presence of a carbon source under conditions effective to express the polynucleotides and produce branched chain fatty alcohols that are secreted into the extracellular environment of the host cell;
(c) collecting the branched chain fatty alcohols; and
(d) blending the branched chain fatty alcohols to make a surfactant composition.
33 : The method of claim 32 , further comprising a polypeptide having fatty alcohol biosynthesis activity, wherein said polypeptide is an alcohol dehydrogenase (EC 1.1.1.1).
33 . (canceled)
34 : The method of claim 32 , wherein said one or more subunits are selected from the group consisting of E1 alpha/beta (decarboxylase), E2 (dihydrolipoyl transacylase), and E3 (dihydrolipoyl dehydrogenase) subunits.
35 : The method of claim 32 , wherein said one or more subunits are selected from the group consisting of E1 alpha/beta (decarboxylase) and E2 (dihydrolipoyl transacylase) subunits.
36 : The method of claim 32 , wherein said polypeptide having fatty aldehyde biosynthesis activity is an acyl-ACP reductase (AAR).
37 : The method of claim 36 , wherein said AAR is an enzyme from Synechococcus elongates.
38 : The method of claim 32 , wherein said polypeptide having fatty aldehyde biosynthesis activity is carboxylic acid reductase (CAR).
39 : The method of claim 32 , wherein the recombinant host cell is an E. coli host cell.
40 : The method of claim 32 , wherein the branched chain fatty alcohols include one or more of saturated or unsaturated C 12 , C 14 and C 16 fatty alcohols.