Biological indicator
This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
1. A composition comprising
a host organism, Geobacillus stearothermophilus comprising; a reporter gene for producing an indicator enzyme; a regulatory gene, wherein the regulatory gene comprises xylR; and
a vehicle comprising a plasmid for inserting the reporter gene and the regulatory gene in the host organism;
the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene;
the insertion sequence comprising nucleotides 2060-4166 of SEQ.ID.NO: 1; wherein the vehicle is taken up by the host organism, the insertion sequence is inserted in the regulatory gene, and the host organism undergoes sporulation.
2. The composition of claim 1 wherein the reporter gene comprises LacZ, bgaB, xylE, cat, gfp, or a mixture of two or more thereof.
3. The composition of claim 1 wherein the indicator enzyme comprises beta-D-galactosidase, beta-D-glucosidase, alpha-D-glucosidase, alkaline phosphatase, acid phosphatase, butyrate esterase, caprylate esterase lipase, chloroamphenicol acetytransferase, catechol-2,3-dioxygenase, myristate lipase, leucine am inopeptidase, valine am inopeptidase, chymotrypsin, phosphohydrolase, alpha-D-galactosidase, alpha-L-arabinofuranosidase, N-acetyl-beta-glucosaminidase, beta-D-cellobiosidase, alanine am inopeptidase, proline am inopeptidase, tyrosine aminopeptidase, phenylalanine aminopeptidase, beta-D-glucuronidase, fatty acid esterase, or a mixture of two or more thereof.
4. The composition of claim 1 wherein the reporter gene comprises bgaB, the reporter gene producing an indicator enzyme comprising beta-galactosidase.
5. The composition of claim 1 wherein the plasmid comprises a circular double-stranded DNA.
6. The composition of claim 1 wherein the plasmid is linear.
7. The composition of claim 1 wherein the size of the plasmid is in the range from about 2000 to about 20000 base pairs.
8. The composition of claim 1 wherein from 1 to about 3000 copies of the plasmid are taken up by a cell of the test organism.
9. The composition of claim 1 wherein the plasmid comprises one or more origins of replication.
10. The composition of claim 1 wherein the plasmid comprises one or more genetic markers.
11. The composition of claim 1 wherein the plasmid comprises one or more multiple cloning sites.
12. The composition of claim 1 wherein the plasmid comprises one or more genes that provide a selective marker to induce the test organism to retain the plasmid.
13. The composition of claim 1 wherein the plasmid comprises a gram negative origin of replication and a gram positive origin of replication.
14. The composition of claim 1 wherein the plasmid comprises an antibiotic resistance gene and/or a gene with exogenous nutritional capability.
15. The composition of claim 1 wherein the plasmid comprises a chloramphenicol, ampicillin or spectinomycin antibiotic gene, and/or a xylose, lactose or amino acid nutritional gene.
16. The composition of claim 1 wherein the reporter gene comprises bgaB, and the indicator enzyme comprises beta-D-galactosidase.
17. The composition of claim 1 wherein the host organism comprises a plasmid construct, and wherein the plasmid construct comprises the sequence set out in SEQ ID No. 1.
18. A composition, comprising
a host organism Geobacillus stearothermophilus comprising;
a reporter gene for producing an indicator enzyme, the indicator enzyme comprising beta-D-galactosidase or alpha-D-glucosidase;
a regulatory gene comprising xylR; and
the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene, the insertion sequence comprising nucleotides 2060-4166 of SEQ.ID.NO: 1; and
a vehicle comprising a plasmid for inserting the reporter gene and the regulatory gene in the host organism; and
wherein the vehicle is taken up by the host organism, the insertion sequence is inserted in the regulatory gene, and the host organism undergoes sporulation.