Method for in vitro differentiation of blood circulating cells into neuronal-like cells and applications thereof
View Patent ↗The present invention relates to a method for in vitro differentiation of a population of blood circulating cells, such as monocytes and preferably pluripotent macrophages derived therefrom, into cells displaying functional and phenotypic neuronal characteristics. The invention further encompasses neuronal-like cells obtainable according to the present method, compositions comprising said cells, and applications thereof.
1. A method for in vitro differentiation of a population of pluripotent macrophages into a population of functional neuronal-like cells, wherein:
1) a population of pluripotent macrophages is grown in a culture medium; and
2) exogenous factors BHA (Butylated hydroxyanisole), RA (Retinoic Acid), IGF-1 (Insulin growth factor-1), and NT-3 (Neurotrophin) are added to the culture medium of step 1),
wherein said pluripotent macrophages express the CD11B and CD14 surface antigen markers.
2. The method according to claim 1 , comprising the prior step of differentiating a population of monocytes into a population of pluripotent macrophages.
3. The method according to claim 1 , comprising the prior step of differentiating a population of monocytes into a population of pluripotent macrophages,
wherein the differentiation of said population of monocytes into said population of pluripotent macrophages is obtained by:
a) growing a population of monocytes in a culture medium; and
b) adding the exogenous factor M-CSF (Macrophage colony-stimulating factor) to the medium of step a).
4. The method according to claim 1 , wherein the exogenous factors of step 2) are sequentially added to the culture medium of step 1), in the following order:
i) BHA (Butylated hydroxyanisole);
ii) RA (Retinoic acid); and
iii) IGF-1 (Insulin growth factor-1) and NT-3 (Neurotrophin-3).
5. The method according to claim 4 , further comprising the step of: iv) adding at least one agent capable of stimulating calcium influx to the medium of step iii).
6. The method according to claim 4 , wherein the BHA (Butylated hydroxyanisole) final concentration in step i) is comprised between about 50 nM and about 200 nM.
7. The method according to claim 4 , wherein the RA (Retinoic Acid) final concentration in step ii) is comprised between about 10 μM and about 20 μM.
8. The method according to claim 4 , wherein:
the IGF-1 (Insulin growth factor-1) final concentration in step iii) is comprised between about 5 ng/ml and about 250 ng/ml; and
the NT-3 (Neurotrophin-3) final concentration in step iii) is of up to about 30 ng/ml.
9. The method according to claim 4 , wherein said BHA (Butylated hydroxyanisole) final concentration is adjusted before step iii) to a concentration comprised between about 50 μM and about 100 μM.