Cell washing device using a wave

1. A method of separating a component from a wash material, comprising:

mixing the wash material with a sample of the component;

transferring the wash material and component, after mixing, to a separating system including a mixing section;

separating the component from the wash material, wherein separating the component includes injecting a directing flow into the mixing section to direct the component towards a collection chamber; and

collecting the component in the collection chamber.

2. The method of claim 1 , wherein the component includes red blood cells.

3. The method of claim 2 , wherein separating the component includes separating red blood cells from the wash material with a standing wave.

4. The method of claim 3 , further comprising generating the standing wave as a standing acoustic wave.

5. The method of claim 4 , wherein the standing acoustic wave is generated on a surface.

6. The method of claim 1 , wherein collecting the component occurs after separating the component from the wash material.

7. A method of separating a component from a wash material, comprising:

mixing the wash material with a sample comprising the component to generate a mixture;

transferring the mixture to a separating system comprising a collection chamber, a first wave generator, and a second wave generator, wherein the first and second wave generators generate a standing surface acoustic wave;

separating the component from the mixture in the separation system, wherein separating the component includes injecting a directing flow into the mixing section to direct the component towards the collection chamber; and

collecting the component in the collection chamber.

8. The method of claim 7 , wherein mixing comprises flowing the sample from a sample source to a mixing chamber, and flowing the wash material from a wash material source to the mixing chamber, wherein the sample and the wash material are mixed to generate the mixture.

9. The method of claim 7 , wherein the transferring the mixture to the separating system comprises pumping the mixture into the separating system with a pump.

10. The method of claim 7 , wherein separating the component from the mixture comprises flowing the mixture through a flow channel relative to the standing surface acoustic wave, wherein the standing surface acoustic moves the component to a component collection path.

11. The method of claim 10 , wherein separating the component from the mixture further comprises flowing the wash material through a wash material collection path, wherein the wash material collection path is different from the component collection path.

12. The method of claim 7 , wherein mixing the wash material with a sample comprising the component includes mixing the wash material with a rejuvenated blood sample, wherein the component comprises red blood cells.

13. A method of separating red blood cells from a wash material, comprising:

transferring a sample of red blood cells and a wash material into a mixing chamber;

mixing the red blood cells with the wash material within the mixing chamber to form a mixture;

pumping the mixture from the mixing chamber to a separation system comprising a flow channel, a first wave generator and a second wave generator, wherein the first and second wave generators generate a standing surface acoustic wave relative to the flow channel;

separating the red blood cells from the wash material by flowing the mixture through the flow channel relative the standing surface acoustic wave and injecting a directing flow into the flow channel to direct the red blood cells towards a red blood cell collection path; and

collecting the red blood cells from the red blood cell collection path.

14. The method of claim 13 , further comprising rejuvenating red blood cells by mixing red blood cells with a rejuvenating solution to generate the sample of red blood cells.

15. The method according to claim 14 , wherein separating further comprises separating the red blood cells from the rejuvenating solution.

16. The method according to claim 13 , wherein mixing comprises mixing the red blood cells and wash material at about 30° C. to about 36° C. for about 2 minutes to about 10 minutes.

17. The method according to claim 13 , further comprising infusing the red blood cells to a patient in need thereof.

18. A system for separating a component from a mixture, comprising:

a mixing chamber; and

a separation apparatus comprising a flow channel positioned relative to a first wave generator and a second wave generator, the first and second wave generators configured to generate a standing surface acoustic wave relative to the flow channel, the flow channel defining a plurality of inlet ports, each of the plurality of inlet ports arranged to inject a directing flow into a flow of the mixture passing through the flow channel,

wherein the mixing chamber is in fluid communication with the separation apparatus.

19. The system according to claim 18 , further comprising a source of a sample and a source of a wash material; wherein the source of a sample and the source of a wash material are in fluid communication with the mixing chamber.

20. The system according to claim 18 , wherein the flow channel comprises a cross-section area of about 0.1 μm 2 to about 100 cm 2 .

21. The system according to claim 18 , wherein the mixing chamber is in communication with the separation apparatus by a conduit that couples the mixing chamber to a first portion of the separation apparatus.

22. The system according to claim 21 , wherein the separation apparatus further comprises a second portion opposite the first portion, wherein the flow channel is bifurcated into a component path and a wash solution path near the second portion, the component path being larger than the wash solution path.

23. The system according to claim 22 , wherein the wash solution path has a diameter about 10% to about 2000% larger than a diameter of the component path.