Influenza virus reassortment method
View Patent ↗Methods for producing reassortant viruses are provided wherein the transcription and/or translation of the hemagglutinin and/or neuraminidase genes are suppressed.
1. A culture comprising:
host cells infected with a first influenza viral strain and a second influenza viral strain,
wherein the host cells are in contact with a nucleic acid inhibitory agent that preferentially reduces the transcription and/or translation of one or more surface antigens of the first or the second influenza viral strain.
2. The culture of claim 1 , wherein the culture is free of animal derived products.
3. The culture of claim 1 , wherein the culture is free of added antibodies to the one or more surface antigens.
4. The culture of claim 1 , wherein the nucleic acid inhibitory agent is a synthetic antisense oligomer.
5. The culture of claim 1 , wherein the nucleic acid inhibitory agent is a phosphorothioate oligomer (PSO).
6. The culture of claim 1 , wherein the nucleic acid inhibitory agent is a phosphorodiamidate morpholino oligomer (PMO).
7. The culture of claim 1 , wherein the nucleic acid inhibitory agent is selected from the group consisting of:
short interfering RNAs (siRNA), double-stranded RNAs (dsRl\fA), micro-RNAs (miRNA), short hairpin RNAs (shRNA), and small interfering DNAs (siDNA).
8. The culture of claim 1 , wherein the culture is an ernbryonated hen egg.
9. The culture of claim 1 , wherein the host cells are mammalian cells or avian cells.
10. The culture of claim 1 , wherein the influenza surface antigens are HA and/or NA.