Non-specific reaction inhibitor
View Patent ↗Disclosed is a non-specific reaction inhibitor for use in an immunological measurement, comprising a complex of an antibody or a fragment of the antibody capable of specifically binding to a non-specific reaction factor, and a polymer. The non-specific reaction inhibitor can inhibit a non-specific reaction which may interfere with the accurate detection or quantification of a trace substance in an immunological measurement method.
1. A method for inhibiting a non-specific reaction in an immunological measurement, said method comprising:
preparing a sample containing an antigen to be measured;
preparing a solution containing a polymer-modified fragment of an antibody specific to a non-specific reaction factor, wherein the polymer is selected from the group consisting of polyethylene glycol, dextran, bovine serum albumin, and polyglutamic acid, the fragment of the antibody is Fab′, and the non-specific reaction factor is selected from the group consisting of IgM, IgG, IgA, IgE, and IgD;
mixing the solution with the sample to react the polymer-modified fragment with the non-specific reaction factor, before an antibody specific to the antigen is reacted with the antigen, and adding the antibody specific to the antigen to the mixture; and
inhibiting a non-specific reaction caused by the non-specific reaction factor.
2. The method according to claim 1 , wherein the molecular weight of the polymer is 200 Da to 1000 kDa.
3. The method according to claim 1 , wherein the polymer-modified antibody fragment is modified by a chemical modification utilizing a thiol, amino, hydroxyl, or carboxyl group, or a biotin-avidin binding.
4. The method according to claim 1 , wherein the immunological measurement is a latex agglutination optical measurement method, an enzyme immunoassay, a nephelometric immunoassay, an enzyme-linked immunosorbent assay, a fluoroimmunoassay, or a radioimmunoassay.
5. A method for inhibiting a non-specific reaction in an immunological measurement, said method comprising:
preparing a sample containing an antigen to be measured;
preparing a solution containing an antibody specific to the antigen and a polymer-modified fragment of an antibody specific to a non-specific reaction factor, wherein the polymer is selected from the group consisting of polyethylene glycol, dextran, bovine serum albumin, and polyglutamic acid, the fragment of the antibody is Fab′, and, the non-specific reaction factor is selected from the group consisting of IgM, IgG, IgA, IgE, and IgD;
mixing the solution with the sample to react the non-specific reaction factor with the polymer-modified fragment; and
inhibiting a non-specific reaction caused by the non-specific reaction factor.
6. The method according to claim 5 , wherein the molecular weight of the polymer is 200 Da to 1000 kDa.
7. The method according to claim 5 , wherein the polymer-modified antibody fragment is modified by a chemical modification utilizing a thiol, amino, hydroxyl, or carboxyl group, or a biotin-avidin binding.
8. The method according to claim 5 , wherein the immunological measurement is a latex agglutination optical measurement method, an enzyme immunoassay, a nephelometric immunoassay, an enzyme-linked immunosorbent assay, a fluoroimmunoassay, or a radioimmunoassay.