Renovation and Repopulation of Decellularized Tissues and Cadaveric Organs by Stem Cells
A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed.
1 .- 23 . (canceled)
24 . A tissue comprising a decellularized organ and a plurality of non-placental cells.
25 . The tissue of claim 24 , wherein said decellularized organ is a placenta.
26 . The tissue of claim 24 , wherein said decellularized organ is a heart.
27 . The tissue of claim 24 , wherein the non-placental cells are stem cells or progenitor cells.
28 . The tissue of claim 27 , wherein the progenitor cells are progenitor cells for neurons, muscle, blood, bone, bone marrow, skin, heart, connective tissue, lung, kidney, liver, or pancreas.
29 . The tissue of claim 28 , wherein said progenitor cells for pancreas are progenitor cells for pancreatic islet cells.
30 . The tissue of claim 27 , wherein the stem cells are mesenchymal stem cells.
31 . The tissue of claim 30 , wherein said mesenchymal stem cells are from bone marrow.
32 . The tissue of claim 30 , wherein said mesenchymal stem cells are from embryonic yolk sac, umbilical cord, fetal skin, adolescent skin, or blood.
33 . The tissue of claim 24 , wherein the non-placental cells are on or more of hematopoietic cells, neuron cells, fibroblast cells, strand cells, or hepatic cells.
34 . The tissue of claim 24 , wherein the non-placental cells are stromal cells, endothelial cells, hepatocytes, or keratinocytes.
35 . The tissue of claim 24 , wherein the non-placental cells are cells that have been differentiated from placental stem cells.
36 . The tissue of claim 35 , wherein the cells that have been differentiated from placental stem cells are adipogenic cells, chondrogenic cells, osteogenic cells, myogenic cells, vasogenic cells, neurogenic cells, or hepatogenic cells.