Systems and methods for detection and quantification of analytes
Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
1. A sample analysis cartridge, comprising:
a housing having internal barriers defining: a plurality of reservoirs, an analysis channel, and an input tunnel,
wherein the plurality of reservoirs comprise a first reservoir at least partially filled with a first liquid volume comprising sample preparation reagents, a second reservoir at least partially filled with a second liquid volume comprising a wash solution, and a third reservoir at least partially filled with a third liquid volume comprising a chemical substrate,
wherein each of the first, second, and third reservoirs is, at least at times, in fluid communication with the analysis channel, and
wherein the input tunnel extends from an aperture at a surface of the housing to the first reservoir; and
a circuit board coupled to the housing, the circuit board comprising an electrochemical sensor disposed within a portion of the analysis channel,
wherein the housing is configured to enclose an internal component and the circuit board, the internal component is disposed on or under the circuit board, features of the internal component and the circuit board define the analysis channel, and features of the internal component define the plurality of reservoirs.
2. The sample analysis cartridge of claim 1 , further comprising a plurality of valves corresponding with the plurality of reservoirs, wherein a valve is positioned at each intersection between one of the plurality of reservoirs and the analysis channel.
3. The sample analysis cartridge of claim 2 , wherein each of the plurality of valves comprises: a phase-changeable material configured to change phases upon the application of heat, and a via positioned on the circuit board and aligned with the phase-changeable material, the via being thermally coupled to a heating element.
4. The sample analysis cartridge of claim 1 , further comprising an absorbent material positioned within the housing at a downstream end of the analysis channel.
5. The sample analysis cartridge of claim 1 , wherein the plurality of reservoirs are in a linear formation.
6. The sample analysis cartridge of claim 1 , wherein the plurality of reservoirs are in a non-linear formation.
7. The sample analysis cartridge of claim 1 , wherein the housing comprises a first cover component and a second cover component, which together enclose the internal component and the circuit board.
8. The sample analysis cartridge of claim 1 , further comprising a membrane spanning a cross-sectional area between the input tunnel and the first reservoir.
9. The sample analysis cartridge of claim 1 , further comprising a sonication component comprising a piezoelectric element, the sonication component aligned with the first reservoir and configured to generate a signal for detection of: flex in the first reservoir, a change in a return sound wave, or a shift in a resonance frequency of the piezoelectric element upon entry of a sample collection device into the first reservoir.
10. The sample analysis cartridge of claim 1 , wherein the sample preparation reagents comprise a plurality of magnetic particles, each magnetic particle having a surface-bound affinity molecule configured to bind to a target analyte.
11. The sample analysis cartridge of claim 10 , wherein the plurality of magnetic particles comprise magnetic particles of at least two sizes, and wherein each size of the magnetic particles has a different surface-bound affinity molecule configured to bind to a different target analyte.
12. The sample analysis cartridge of claim 10 , wherein the sample preparation reagents further comprise:
a plurality of detector agents; and
a plurality of agents that facilitate binding between the target analytes, the surface-bound affinity molecules, and the detector agents within the first reservoir.
13. The sample analysis cartridge of claim 12 , wherein the detector agents comprise oxidizing enzymes for oxidizing the chemical substrate of the third reservoir.
14. The sample analysis cartridge of claim 10 , further comprising a membrane spanning a cross-sectional area between the input tunnel and the first reservoir, wherein a plurality of competitive binding agents are dry-stored on or in the membrane, each competitive binding agent comprising a pre-bound target analyte bound to a signaling agent, and wherein the sample preparation reagents in the first reservoir further comprise a plurality of agents that facilitate binding of: the surface-bound affinity molecules to the target analytes, and the surface-bound affinity molecules to the competitive binding agents.
15. The sample analysis cartridge of claim 14 , wherein the signaling agent comprises an oxidizing enzyme for oxidizing the chemical substrate.
16. The sample analysis cartridge of claim 1 , wherein the housing defines a self-contained unit configured to store, during and after analyte detection, all collected sample, liquids, and reagents needed to detect a target analyte.
17. A kit comprising:
the sample analysis cartridge of claim 1 ; and
a sample collection device sized to fit at least partially within the input tunnel.
18. The kit of claim 17 , wherein the sample collection device is configured to retain a biological sample collected from blood, urine, inside a mouth, or inside a nose.
19. The kit of claim 17 , wherein the input tunnel and the sample collection device have complementary locking features configured to securely lock the sample collection device in place within the input tunnel.
20. The kit of claim 17 , wherein the sample collection device is sized and configured to form a liquid-tight seal with the input tunnel.
21. A sample analysis cartridge, comprising:
a housing having internal barriers defining: a plurality of reservoirs, an analysis channel, and an input tunnel,
wherein the plurality of reservoirs comprise a first reservoir at least partially filled with a first liquid volume comprising sample preparation reagents, a second reservoir at least partially filled with a second liquid volume comprising a wash solution, and a third reservoir at least partially filled with a third liquid volume comprising a chemical substrate, wherein the sample preparation reagents comprise a plurality of magnetic particles, each magnetic particle having a surface-bound affinity molecule configured to bind to a target analyte,
wherein each of the first, second, and third reservoirs is, at least at times, in fluid communication with the analysis channel, and
wherein the input tunnel extends from an aperture at a surface of the housing to the first reservoir;
a circuit board coupled to the housing, the circuit board comprising an electrochemical sensor disposed within a portion of the analysis channel; and
a membrane configured to span a cross-sectional area between the input tunnel and the first reservoir, wherein a plurality of competitive binding agents are dry-stored on or in the membrane, each competitive binding agent comprising a pre-bound target analyte bound to a signaling agent, and wherein the sample preparation reagents in the first reservoir further comprise a plurality of agents that facilitate binding of: the surface-bound affinity molecules to the target analytes, and the surface-bound affinity molecules to the competitive binding agents.
22. The sample analysis cartridge of claim 21 , further comprising a plurality of valves corresponding with the plurality of reservoirs, wherein a valve is positioned at each intersection between one of the plurality of reservoirs and the analysis channel.
23. The sample analysis cartridge of claim 22 , wherein each of the plurality of valves comprises: a phase-changeable material configured to change phases upon the application of heat, and a via positioned on the circuit board and aligned with the phase-changeable material, the via being thermally coupled to a heating element.
24. The sample analysis cartridge of claim 21 , further comprising an absorbent material positioned within the housing at a downstream end of the analysis channel.
25. The sample analysis cartridge of claim 21 , wherein:
the housing comprises a first cover component and a second cover component, which together enclose an internal component and the circuit board;
the internal component is disposed on or under the circuit board;
features of the internal component and the circuit board define the analysis channel; and
features of the internal component define a plurality of reservoirs.
26. The sample analysis cartridge of claim 21 , further comprising a sonication component comprising a piezoelectric element, the sonication component aligned with the first reservoir and configured to generate a signal for detection of: flex in the first reservoir, a change in a return sound wave, or a shift in a resonance frequency of the piezoelectric element upon entry of a sample collection device into the first reservoir.
27. The sample analysis cartridge of claim 21 , wherein the signaling agent comprises an oxidizing enzyme for oxidizing the chemical substrate.
28. A kit comprising:
the sample analysis cartridge of claim 21 ; and
a sample collection device sized to fit at least partially within the input tunnel.
29. The kit of claim 28 , wherein the sample collection device is configured to retain a biological sample collected from blood, urine, inside a mouth, or inside a nose.
30. The kit of claim 28 , wherein the input tunnel and the sample collection device have complementary locking features configured to securely lock the sample collection device in place within the input tunnel.