Genetic loci associated with head smut resistance in maize
Head smut is one of the most devastating diseases in maize, causing severe yield loss worldwide. The present invention describes the fine-mapping of a major QTL conferring resistance to head smut. Markers useful for breeding, and methods for conferring head smut resistance are described. Nucleic acid sequence from the genetic locus conferring head smut resistance is disclosed. Genes encoding proteins conferring head smut resistance are disclosed.
1. A recombinant DNA construct comprising an isolated polynucleotide operably linked to at least one heterologous regulatory sequence, wherein said isolated polynucleotide comprises:
a. a nucleotide sequence encoding a polypeptide conferring or improving resistance to head smut, wherein the polypeptide has the amino acid sequence as set forth in SEQ ID NO:32; or
b. a nucleotide sequence capable of encoding a polypeptide conferring or enhancing resistance to headsmut, wherein said nucleotide sequence is as set forth in SEQ ID NO:31.
2. A maize cell comprising the recombinant DNA construct of claim 1 .
3. A method for producing a maize plant, said method comprising:
a. transforming a maize plant cell with the recombinant DNA construct of claim 1 ; and
b. regenerating a maize plant from the transformed plant cell.
4. A maize plant comprising the recombinant DNA construct of claim 1 .
5. A maize seed comprising the recombinant DNA construct of claim 1 .
6. A method of conferring or improving resistance to head smut, said method comprising;
a. transforming a plant with a DNA construct comprising a nucleotide sequence encoding a polypeptide conferring or improving resistance to head smut, wherein the polypeptide has an amino acid sequence with at least 95% sequence identity to SEQ ID NO:32, thereby conferring or improving resistance to head smut: and
b. comparing the transformed plant to another plant lacking the DNA construct for improved resistance to head smut.
7. A method of altering the level of expression of a protein capable of conferring resistance to head smut in a maize cell, said method comprising:
a. transforming a maize cell with a DNA construct-comprising a nucleotide sequence encoding a polypeptide conferring or improving resistance to head smut wherein the polypeptide has an amino acid sequence of at least 95% sequence identity to SEQ ID NO:32;
b. growing the transformed maize cell under conditions that are suitable for expression of the recombinant DNA construct, wherein expression of the recombinant DNA construct results in production of altered levels of the protein that confers or improves resistance to head smut in the transformed maize cell when compared to levels of expression in a wild-type maize plant: and
c. comparing the transformed maize cell to another plant lacking the DNA construct for improved resistance to head smut.
8. A method of altering the level of expression of a protein capable of conferring resistance to head smut in a maize plant, said method comprising:
a. transforming a maize plant cell with a DNA construct comprising a nucleotide sequence encoding a polypeptide conferring or improving resistance to head smut, wherein the polypeptide has an amino acid sequence with at least 95% sequence identity to SEQ ID NO:32;
b. regenerating a transformed maize plant from the transformed maize plant cell;
c. growing the transformed maize plant under conditions that are suitable for expression of the recombinant DNA construct wherein expression of the recombinant DNA construct results in production of altered levels of the protein that confers or improves resistance to head smut in the transformed maize plant when compared to levels of expression in a wild-type maize plant having resistance to head smut; and
d. assaying the resulting maize plant for improved head smut resistance compared to a plant ladling the DNA construct.
9. A modified plant comprising the recombinant DNA construct of claim 1 .
10. The modified plant of claim 9 , wherein the nucleotide sequence comprises SEQ ID NO: 31.