Turkey herpesvirus vectored recombinant containing avian influenza genes
The present invention provides a recombinant turkey herpesvirus modified by the presence of the cDNA encoding the hemagglutinin protein of avian influenza virus under a promoter. A poultry vaccine comprising the recombinant turkey herpesvirus described in the present invention can induce serological responses that may be easily detected by the hemagglutination inhibition assay but not by commercially available diagnostic ELISA kits; thus enabling easy differentiation between vaccination and field infection.
1. A recombinant turkey herpesvirus comprising a hemagglutinin gene of avian influenza virus and a cytomegalovirus immediate early promoter, wherein said hemagglutinin (HA) gene is under control of said cytomegalovirus immediate early promoter, and wherein said HA gene is obtained from an H7 subtype of avian influenza virus.
2. The recombinant turkey herpesvirus according to claim 1 , wherein said hemagglutinin gene and said cytomegalovirus immediate early promoter are present in a non-essential region of the turkey herpesvirus genome.
3. The recombinant turkey herpesvirus according to claim 2 , wherein said non-essential region is between UL45 and UL46 of the turkey herpesvirus genome.
4. A poultry vaccine comprising the recombinant turkey herpesvirus according to claim 1 .
5. The poultry vaccine according to claim 4 , said vaccine further comprising a non-recombinant virus.
6. The poultry vaccine according to claim 5 , wherein said non-recombinant virus is a serotype 1 Marek's disease virus (MDV).
7. The poultry vaccine according to claim 5 , wherein said non-recombinant virus is a serotype 2 Marek's disease virus (MDV).