IP Library Granted Patent US 10,387,301
Granted Patent B2
US 10,387,301 · App. 14/556,213 · Granted Aug 20, 2019

High-capacity storage of digital information in DNA

Inventors: Nick Goldman (Royston, GB); John Birney (London, GB)
Assignee: European Molecular Biology Laboratory
G06F12/023B82Y10/00G06N3/123G16B30/00G16B50/40G16B50/50G06F2212/1032
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Quick Facts
Patent No.
US 10,387,301
App. No.
14/556,213
Granted
Aug 20, 2019
Kind
B2
Abstract

A method for storage of an item of information ( 210 ) is disclosed. The method comprises encoding bytes ( 720 ) in the item of information ( 210 ), and representing using a schema the encoded bytes by a DNA nucleotide to produce a DNA sequence ( 230 ). The DNA sequence ( 230 ) is broken into a plurality of overlapping DNA segments ( 240 ) and indexing information ( 250 ) added to the plurality of DNA segments. Finally, the plurality of DNA segments ( 240 ) is synthesized ( 790 ) and stored ( 795 ).

Claims (18)

1. A method of storing an item of information in a plurality of DNA segments, the method comprising:

encoding bytes in an item of information stored in a first computer file as a DNA sequence data using a representation schema to represent the encoded bytes as at least one DNA nucleotide datum in the DNA sequence data;

splitting the DNA sequence data into a plurality of overlapping DNA segments data, wherein the plurality of overlapping DNA segments data build in a redundancy of storage of the items of information;

adding indexing information to the plurality of DNA segments data, the indexing information indicating a position in the DNA sequence data of any one nucleotide datum in any one of the plurality of DNA segments data;

synthesizing, by providing the plurality of DNA segments data as input to a computer-controlled DNA synthesis platform, a plurality of DNA segments.

2. The method of claim 1 , further including the addition of adapters data to the DNA segments data.

3. The method of claim 1 using a base-3 scheme for encoding the bytes.

4. The method of claim 1 , wherein the representation schema used is designed such that adjacent ones of the DNA nucleotide data are different.

5. The method of claim 1 , further comprising adding a parity-check to the indexing information.

6. The method of claim 1 , wherein alternate ones of the DNA segments data are reverse complemented.

7. The method of claim 1 , wherein the representation schema used is designed to avoid long, self-reverse complementary DNA segments data.

8. The method of claim 1 , further comprising:

storing the synthesized DNA segments in one or more DNA archives.

9. A plurality of DNA segments that store an item of information, wherein the plurality of DNA segments data are produced by a method comprising:

encoding bytes in an item of information, stored in a first computer file, as a DNA sequence data using a representation schema to represent the encoded bytes as at least one DNA nucleotide datum in the DNA sequence data;

splitting the DNA sequence data into a plurality of overlapping DNA segments data, wherein the plurality of overlapping DNA segments data build in a redundancy of storage of the items of information;

augmenting indexing information to the plurality of DNA segments data, the indexing information indicating a position in the DNA sequence data of any one nucleotide datum in any one of the plurality of DNA segments data; and

synthesizing, by providing the plurality of DNA segments data as input to a computer-controlled DNA synthesis platform, a plurality of DNA segments.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 16, 2015
From: GOLDMAN, NICK; BIRNEY, JOHN
To: EUROPEAN MOLECULAR BIOLOGY LABORATORY
Reel/Frame 035844/0374 →
Continuity (3)
Continuation PCTEP2013061300 · May 31, 2013
Provisional Application 61654295 · Jun 1, 2012
Related Publication 20150261664A1 · Sep 17, 2015
Cited By (3)
US 12,236,354 US 12,437,841 US 12,511,228