METHODS AND COMPOSITIONS FOR REGULATING MUSASHI FUNCTION
The invention generally features compositions and methods for detecting and regulating cell proliferation, potentiation, and differentiation in a population of cells. In particular, compositions and methods are provided for modulating the activity of Musashi proteins. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.
1 . An isolated antibody, wherein the antibody specifically binds to an epitope comprising at least 10 amino acids of an antigenic peptide selected from the group consisting of:
a. about amino acids 311-320 of SEQ ID NO:11;
b. about amino acids 271-280 of SEQ ID NO:12;
c. about amino acids 311-320 of SEQ ID NO:13;
d. about amino acids 291-300 of SEQ ID NO:14;
e. about amino acids 291-300 of SEQ ID NO:15; and
f. about amino acids 351-360 of SEQ ID NO:16.
2 . The isolated antibody of claim 1 , wherein the antigenic peptide comprises an amino acid sequence having about 85% identity to a sequence selected from the group consisting of:
a. about amino acids 311-320 of SEQ ID NO:11;
b. about amino acids 271-280 of SEQ ID NO:12;
c. about amino acids 311-320 of SEQ ID NO:13;
d. about amino acids 291-300 of SEQ ID NO:14;
e. about amino acids 291-300 of SEQ ID NO:15; and
f. about amino acids 351-360 of SEQ ID NO:16.
3 . The isolated antibody of claim 1 , wherein the antigenic peptide has been post-translationally modified.
4 . The isolated antibody of claim 1 , wherein the antibody is selected from the group consisting of a single-chain antibody, an antibody fragment, a chimeric antibody, or a humanized antibody.
5 . A composition, the composition comprising an antibody of claim 1 .
6 . A method of detecting post-translational status of Musashi protein comprising:
a. contacting a sample with an antibody, wherein the antibody specifically binds to an epitope comprising at least 10 amino acids of an antigenic peptide selected from the group consisting of:
i. about amino acids 311-320 of SEQ ID NO:11;
ii. about amino acids 271-280 of SEQ ID NO:12;
iii. about amino acids 311-320 of SEQ ID NO:13;
iv. about amino acids 291-300 of SEQ ID NO:14;
v. about amino acids 291-300 of SEQ ID NO:15; and
vi. about amino acids 351-360 of SEQ ID NO:16; and,
b. detecting the post-translational status of Musashi protein.
7 . The method of claim 6 , wherein the sample is a biological sample selected from the group consisting of blood, serum, cells and tissue.
8 . A method of modulating Musashi protein activity comprising contacting a sample with an antibody that modulates Musashi activity, wherein the antibody specifically binds to an epitope comprising at least 10 amino acids of an antigenic peptide selected from the group consisting of:
a. about amino acids 311-320 of SEQ ID NO:11;
b. about amino acids 271-280 of SEQ ID NO:12;
c. about amino acids 311-320 of SEQ ID NO:13;
d. about amino acids 291-300 of SEQ ID NO:14;
e. about amino acids 291-300 of SEQ ID NO:15; and
f. about amino acids 351-360 of SEQ ID NO:16.
9 . The method of claim 8 , wherein the antibody modulates Musashi protein by inhibiting one or more of the biological activities of Musashi protein.