Microscope apparatus
View Patent ↗A plurality of specimens or a plurality of targets in a specimen are accurately examined. A microscope apparatus includes a light source; an image-generating unit generating an image of a specimen; a control unit controlling the light source and the image-generating unit depending on a predetermined irradiation condition or a predetermined image generation condition; an image-analyzing unit analyzing the image generated by the image-generating unit to extract targets; and a condition-changing unit changing the irradiation condition and/or the image generation condition based on a difference between an actual luminance of each of the extracted targets in the image and a desired luminance such that the actual luminance of the target in the image satisfies the desired luminance. If the irradiation condition and/or the image generation condition is changed, the control unit redrives the light source and the image-generating unit depending on the changed irradiation condition and/or image generation condition.
1. A control method for a microscope apparatus which comprises a scanner, a light source that emits illumination light, and an image-generating unit including an image sensor, the method comprising:
performing precapturing to capture a first image of a specimen, wherein the precapturing comprises controlling the light source to operate under a predetermined irradiation condition to emit illumination light, scanning the illumination light emitted by the light source operating under the predetermined irradiation condition across the specimen with the scanner, and controlling the image-generating unit to operate under a predetermined image generation condition to generate the first image of the specimen by detecting fluorescence emitted from the specimen when the specimen is scanned with the illumination light emitted from the light source under the predetermined irradiation condition;
analyzing the generated first image to extract a plurality of examination targets of the specimen in the first image, the first image being a single image, and to detect an actual luminance of each of the extracted examination targets in the same single first image;
changing at least one of the irradiation condition and the image generation condition for each of the examination targets in the same single first image based on a difference between the actual luminance of each of the extracted examination targets detected in the same single first image and a desired luminance; and
performing main capturing to capture a second image of the specimen which includes the examination targets, wherein the main capturing comprises redriving the light source, scanning illumination light emitted by the redriven light source across the specimen with the scanner, and redriving the image-generating unit to generate the second image, which is a single image, wherein, in the main capturing, the light source and the image-generating unit are redriven under the changed at least one of the irradiation condition and the image generation condition, and
wherein, in the changing of the at least one of the irradiation condition and the image generation condition, the changed at least one of the irradiation condition and the image generation condition is set such that an actual luminance of each of the examination targets in the same single second image satisfies the desired luminance.
2. The method according to claim 1 , wherein in the performing of the main capturing, the at least one of the irradiation condition and the image generation condition is changed for each of the examination targets, for each pixel in the second image, or for each region containing a plurality of pixels.
3. The method according to claim 1 , wherein in the changing of the at least one of the irradiation condition and the image generation condition, the at least one of the irradiation condition and the image generation condition is changed based on a table or a calculation formula that associates the examination targets with the desired luminance.
4. The microscope apparatus according to claim 1 , wherein the irradiation condition is a laser power which indicates an intensity of a laser light.
5. The microscope apparatus according to claim 1 , wherein the image generation condition is a gain by which an image signal is amplified.