Methods of using regenerative cells in the treatment of erectile dysfunction
View Patent ↗Cells present in adipose tissue are used to treat patients, including patients with PVD and related diseases or disorders. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Accordingly, in a preferred method, cells present in adipose tissue are placed directly into a recipient along with such additives necessary to promote, engender or support a therapeutic benefit.
1. A method of treating erectile dysfunction in a subject, comprising:
identifying a subject with erectile dysfunction; and
providing to the subject an amount of a concentrated population of adipose-derived regenerative cells comprising stem cells and endothelial progenitor cells that is sufficient to ameliorate said physiologic erectile dysfunction, wherein said concentrated population of adipose-derived regenerative ells is obtained by acquiring an adipose tissue sample, separating away adipocytes and connective tissue, and washing and concentrating said adipose-derived regenerative cells.
2. The method of claim 1 , further comprising providing an additive to said concentrated population of adipose-derived regenerative cells.
3. The method of claim 1 , wherein said subject is human.
4. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is provided to said subject in multiple doses.
5. The method of claim 1 , further comprising providing one or more angiogenic factors to said subject or to said concentrated population of adipose-derived regenerative cells.
6. The method of claim 1 , further comprising providing one or more immunosuppressive drugs to said subject or to said concentrated population of adipose-derived regenerative cells.
7. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is provided to said subject via at least one of an intravenous, intra-muscular or subcutaneous administration route.
8. The method of claim 1 , wherein the concentrated population of adipose-derived regenerative cells is provided on a scaffold.
9. The method of claim 8 , wherein the scaffold is resorbable in vivo.
10. The method of claim 8 , wherein said scaffold is a unit of adipose tissue.
11. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is autologous.
12. The method of claim 1 , further comprising measuring blood flow by a method selected from the group consisting of: duplex ultrasonography, angiography, and magnetic resonance angiography.
13. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is isolated in a system comprising:
a tissue collection chamber that is configured to receive unprocessed adipose tissue that is removed from a subject, wherein said tissue collection chamber is defined by a closed system;
a first filter that is disposed within said tissue collection chamber, wherein said first filter is configured to retain a first component of said unprocessed adipose tissue and pass a second component of said unprocessed adipose tissue, such that said first filter substantially separates said first component from said second component, and wherein said first component comprises a cell population that comprises adipose-derived stem cells, and said second component comprises lipid, mature adipocytes, and saline;
a processing chamber, which is configured to receive said first component comprising said cell population that comprises adipose-derived stem cells from said tissue collection chamber, wherein said processing chamber is within said closed system;
a conduit configured to allow passage of said first component comprising said cell population comprising adipose-derived stem cells from said tissue collection chamber to said processing chamber while maintaining a closed system;
a cell concentrator disposed within said processing chamber, which is configured to facilitate the concentration of said first component comprising said cell population that comprises adipose-derived stem cells so as to obtain a concentrated population of cells that comprises adipose-derived stem cells, wherein said cell concentrator comprises a centrifugation device or a filter; and
an outlet configured to allow the aseptic removal of said concentrated population of cells that comprise adipose-derived stem cells.
14. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is not cultured.
15. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is cultured.
16. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is cryopreserved.
17. The method of claim 1 , wherein said concentrated population of adipose-derived regenerative cells is administered via a catheter.