Assay for detection of human parvovirus nucleic acid
View Patent ↗Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.
1. An amplification oligomer combination for amplifying human parvovirus genotypes 1, 2, and 3, the oligomer combination comprising:
a) at least one primer oligomer member comprising a target binding sequence that is SEQ ID NO:48 or SEQ ID NO:50;
b) a first promoter-based oligomer member comprising a sequence that is SEQ ID NO:73; and
c) a second promoter-based oligomer member comprising a sequence that is SEQ ID NO:78.
2. An amplification oligomer combination for amplifying human parvovirus genotypes 1, 2, and 3, the oligomer combination comprising at least one primer oligomer member and at least one promoter-based oligomer member, wherein the at least one primer oligomer member and the at least one promoter-based oligomer member hybridize to opposing strands of a human parvovirus genotype 1, 2 or 3 nucleic acid or amplicon thereof for transcription mediated amplification of the target nucleic acid or amplicon thereof between the hybridized primer and promoter-based oligomer members, wherein
A) the at least one promoter-based oligomer member is a first promoter-based oligomer member that is SEQ ID NO:76 and a second promoter-based oligomer member that is SEQ ID NO:81;
B) the at least one promoter-based oligomer member is selected from the group consisting of SEQ ID NOS:58, 63, 67, 68, 73, 78, 82, and combinations thereof;
C) the at least one primer oligomer member is SEQ ID NO:50 and the at least one promoter-based oligomer member is a first promoter-based oligomer member that is SEQ ID NO:73 and a second promoter-based oligomer member that is SEQ ID NO:78; or
D) the at least one primer oligomer member is SEQ ID NO:47 and the at least one promoter-based oligomer member is selected from the group consisting of SEQ ID NOs:73, 76, 78, 81 and combinations thereof.
3. The amplification oligomer combination of claim 1 , wherein the at least one primer oligomer member comprises the target-binding sequence that is SEQ ID NO:50.
4. The amplification oligomer combination of claim 1 , wherein the at least one primer oligomer member comprises the target-binding sequence that is SEQ ID NO:48.
5. The amplification oligomer combination of claim 1 , wherein the at least one primer oligomer member is SEQ ID NO:47.
6. The amplification oligomer combination of claim 1 , wherein the at least one primer member is selected from the group consisting of SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50 and combinations thereof.
7. A reaction mixture for performing a transcription mediated amplification reaction, wherein the reaction mixture comprises:
a) an amplification oligomer combination comprising:
i) at least one primer oligomer member comprising a target binding sequence that is SEQ ID NO:48 or SEQ ID NO:50;
ii) a first promoter-based oligomer member comprising a sequence that is SEQ ID NO:73; and
iii) a second promoter-based oligomer member comprising a sequence that is SEQ ID NO:78;
and
b) an enzyme comprising an activity selected from the group consisting of reverse transcriptase activity, RNA polymerase activity, and RNase H activity.
8. The reaction mixture of claim 7 , wherein the enzyme comprises reverse transcriptase activity and RNase H activity.
9. The amplification oligomer combination of claim 2 , wherein the at least one promoter-based oligomer member comprises a blocking moiety at its 3′ end.