Security system and method of marking an inventory item and/or person in the vicinity
A method of marking an inventory item includes providing an activatable smoke generator and a reservoir for holding a smoke fluid and adapted to provide a flow of smoke fluid to the generator. The reservoir contains a smoke fluid including a carrier nucleic acid having a uniquely identifiable sequence, and upon activation of the smoke generator, marker smoke is generated and targeted to flow over the inventory item. The method further includes activating the smoke generator to produce the marker smoke including the carrier nucleic acid so as to cause the marker smoke to flow over the inventory item and thereby to detectably mark the inventory item with carrier nucleic acid. The invention provides methods for stably binding and immobilizing deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate. The alkaline conditions are produced by mixing the deoxyribonucleic acid with an alkaline solution having a pH of about 9.0 or higher, and contacting the deoxyribonucleic acid to the substrate. The immobilized DNA can be used as a taggant and can be used in combination with other detectable taggants, such as optical reporters.
1. A method of marking an inventory item consisting of:
providing a water-based fog generator, providing a reservoir for holding a marker fluid and adapted to provide the flow of marker fluid to the fog generator; the reservoir containing marker fluid consisting of i) a double-stranded carrier nucleic acid; ii) a double-stranded DNA taggant having a uniquely identifiable sequence, wherein said double-stranded DNA taggant is unencapsulated; and iii) deionized water;
activating the water-based fog generator to produce a non-electrostatically charged marker fluid fog so as to cause the marker fluid fog to flow over the inventory item and thereby to detectably mark the inventory item with the unencapsulated double-stranded DNA taggant, wherein the unencapsulated double-stranded DNA taggant is less than one part per then thousand by weight of the double-stranded carrier DNA and is detected by real-time PCR.
2. The method of claim 1 , wherein uniquely identifiable sequence of the unencapsulated double-stranded DNA taggant is a sequence of from about 25 bases to about 10,000 bases in length.
3. The method of claim 2 , wherein the uniquely identifiable sequence of the unencapsulated double-stranded DNA taggant is a sequence of from about 50 bases to about 5,000 bases in length.
4. The method of claim 3 , wherein the uniquely identifiable sequence of the unencapsulated double-stranded DNA taggant is a sequence of from about 75 bases to about 500 bases in length.
5. The method of claim 1 , wherein the unencapsulated double-stranded DNA taggant having a uniquely identifiable sequence is less than one part per hundred thousand by weight of the carrier nucleic acid.
6. The method of claim 5 , wherein the unencapsulated double-stranded DNA taggant having a uniquely identifiable sequence is less than one part per million by weight of the carrier nucleic acid.
7. The method of claim 1 , further comprising detecting the unencapsulated double-stranded DNA taggant of the detectably marked inventory item and thereby authenticating the detectably marked inventory item.
8. The method of claim 7 , wherein the detection of the unencapsulated double-stranded DNA taggant comprises a PCR amplification step.