Method and device for marking fibrous materials
A method of marking fibers, wherein the method includes providing a plurality of fibers; depositing a marker onto at least a portion of the fibers, the depositing being performed with a delivery mechanism comprising one or more outlets; and thereby marking the fibers. Also provided is a device for marking fibers, including a transport system adapted to transport fibers in a direction of a marker delivery apparatus positioned along the transport system; the delivery apparatus includes one or more outlets, adapted to deposit a solution of the marker through the outlets onto at least a portion of the fibers; and thereby marking the fibers. Authentication of a fibrous material using the marking method of the invention followed obtaining a sample of the marked fibers and assaying the sample for the presence of the nucleic acid marker; and thereby determining whether the fibrous material is authentic or counterfeit.
1. A method of DNA marking raw cotton fiber lint, the method comprising:
providing a plurality of raw cotton fiber lint in a ginning process;
forcing the raw cotton fiber lint through a duct by forced air;
emitting a DNA marker solution consisting of a double stranded DNA marker of a known sequence and reverse osmosis filtered water at a double stranded DNA marker concentration of 1 attogram per milliliter of filtered water to 1 microgram per milliliter of filtered water into the duct via a delivery mechanism comprising one or more outlets in fluid communication with the duct;
wherein the DNA marker solution is evenly deposited on the raw cotton fiber lint; and
wherein the DNA marker solution is delivered at a user defined flow rate through the one or more outlets into the duct.
2. A method of authenticating a cotton material comprising:
separating cotton fiber lint from cotton seeds, thereby creating a plurality of raw cotton fiber lint;
forcing the raw cotton fiber lint through a duct by forced air;
evenly depositing a DNA marker solution consisting of a double stranded DNA marker of a known sequence and reverse osmosis filtered water at a double stranded DNA marker concentration of 1 attogram per milliliter of filtered water to 1 microgram per milliliter of filtered water onto the raw cotton fiber lint passing through the duct, the depositing being performed with a delivery mechanism comprising spray nozzles spraying the DNA marker solution into the duct at a user defined flow rate;
thereby producing DNA marked raw cotton fiber lint; and
obtaining a sample of DNA marked raw cotton fiber lint and assaying the sample of DNA marked raw cotton fiber lint for the presence of the DNA marker via polymerase chain reaction (PCR) or isothermal amplification to create an amplified product;
and subjecting the amplified product to a specific sequence detection method thereby determining whether the DNA marked raw cotton fiber lint is authentic or counterfeit.
3. The method as defined in claim 1 , wherein the DNA marker is comprised of DNA and an optical reporter.
4. The method as defined in claim 1 , wherein droplets of DNA marker solution are mixed with the raw cotton fiber lint in a turbulent airstream in the duct.
5. The method as defined in claim 4 , wherein the droplets are less than 200 microns in diameter.
6. The method as defined in claim 1 , wherein the DNA marker is between 50 base pairs and 500 base pairs in length.
7. The method of claim 1 , wherein the DNA is a natural DNA sequence.
8. The method of claim 1 , wherein the DNA is an artificial DNA sequence.
9. A method of DNA marking cotton fibers, the method comprising:
providing a plurality of raw cotton fiber lint in a ginning process;
forcing the raw cotton fiber lint through a lint flue by a forced air stream;
filtering water through a reverse osmosis filter;
mixing a double stranded DNA marker of a known sequence with an alkali activator to create an alkali activated DNA marker and reverse osmosis filtered water at an alkali activated DNA marker concentration of 1 attogram per milliliter of filtered water to 1 microgram per milliliter of filtered water to form an alkaline activated DNA marker solution consisting only of alkali activated DNA, alkali activator and reverse osmosis filtered water; and
emitting the alkaline activated DNA marker solution into the lint flue forced air stream via a delivery mechanism comprising one or more spray nozzles, whereby the alkaline activated DNA marker solution is mixed in the forced air stream to, thereby, coat the alkaline activated DNA marker solution on the raw cotton fiber lint to mark the raw cotton fiber lint.