ASSAY DEVICES COMPRISING A POLY(ACID) MEMBRANE, AND METHODS USING THE SAME
Assay devices that include a poly(acid) membrane are provided. Aspects of the devices include a solid support and a poly(acid) membrane on a surface of the support, where the poly(acid) membrane includes an affinity element. In using the assay devices, a sample is contacted with the poly(acid) membrane and then a signal is obtained from the membrane. Also provided are kits that find use in practicing the methods described herein. The compositions and methods described herein find use in a variety of different applications, including analyte detection applications.
1 . An assay device, the device comprising:
a solid support; and
a poly(acid) membrane positioned on a surface of the solid support, wherein the poly(acid) membrane comprises an affinity element.
2 . The device according to claim 1 , wherein the poly(acid) membrane comprises a poly(acid) component adsorbed to a surface of a porous membrane support.
3 . The device according to claim 2 , wherein the poly(acid) component comprises a film.
4 . The device according to claim 2 , wherein the poly(acid) component comprises poly(acid) brushes.
5 . The spin column according to claim 1 , wherein the affinity element comprises a non-specific affinity element.
6 . The device according to claim 5 , wherein the non-specific affinity element comprises metal ion chelating ligand complexed with a metal ion.
7 . The spin column according to claim 1 , wherein the affinity element comprises a specific affinity element.
8 . The device according to claim 7 , wherein the specific affinity element comprises an antibody or binding fragment thereof.
9 . The spin column according to claim 1 , wherein the device further comprise a second poly(acid) membrane on a surface of the solid support, wherein the second poly(acid) membrane lacks an affinity element.
10 . The device according to claim 1 , wherein the solid support is an elongated structure.
11 . The device according to claim 10 , wherein the elongated structure comprises a cap at a first end.
12 . The device according to claim 11 , wherein the cap is a screw cap.
13 . The device according to claim 11 , wherein the device is present in a vial having a first end configured to mate with the cap.
14 . A method of assaying a sample, the method comprising:
contacting the sample with a device according to claim 1 ; and
obtaining a signal from the poly(acid) membrane to assay the sample.
15 . The method according to claim 14 , wherein the sample is passed through the poly(acid) membrane.
16 . The method according to claim 14 , wherein the method comprises washing unbound sample components from the poly(acid) membrane.
17 . The method according to claim 14 , wherein the method further comprises exposing the sample contacted poly(acid) membrane to a signal producing system.
18 . The method according to claim 14 , wherein the method is a method of assaying the sample for the presence of an analyte.
19 . The method according to claim 18 , wherein the analyte is a diagnostic analyte.
20 . The method according to claim 18 , wherein the analyte is a non-diagnostic analyte.
21 . A kit comprising:
a device according to claim 1 ; and
a vial configured to house the solid support and a volume of a liquid sample.
22 - 23 . (canceled)