Biomass productivity regulator
The disclosure generally relates to methods and materials for modulating cell productivity. In particular, the present disclosure provides polynucleotides and polypeptides that when overexpressed in microorganisms result in increased in productivity, such as increased biomass productivity. Also disclosed are methods of using the polynucleotides and polypeptides to modulate or increase productivity of host cells such as, for example, algal or heterokont cells. Genetically engineered host cells, such as algal and heterokont cells having increased biomass productivity and bioproducts derived from such host cells are also disclosed.
1. An isolated or recombinant nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide that comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:4, wherein the nucleic acid molecule is operably linked to a heterologous nucleic acid sequence, wherein the encoded polypeptide belongs to pfam PF00249 and has DNA binding and transcription factor activity, and further wherein elevated expression of the isolated or recombinant nucleic acid molecule in a recombinant Nannochloropsis cell results in increased biomass productivity, increased lipid productivity, or increased growth rate of the recombinant Nannochloropsis cell as compared to a control recombinant Nannochloropsis cell that does not express the isolated or recombinant nucleic acid molecule.
2. The isolated or recombinant nucleic acid molecule according to claim 1 , wherein the heterologous nucleic acid sequence comprises a regulatory element.
3. The isolated or recombinant nucleic acid molecule according to claim 1 , wherein the polypeptide comprises an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2.
4. A vector comprising the isolated or recombinant nucleic acid molecule according to claim 1 .
5. A recombinant algal or heterokont cell comprising the isolated or recombinant nucleic acid molecule of claim 2 .
6. The recombinant algal or heterokont cell according to claim 5 , wherein the recombinant algal or heterokont cell is a recombinant heterokont cell.
7. The recombinant heterokont cell according to claim 6 , wherein the recombinant heterokont cell is of a genus selected from the group consisting of Labryinthula, Labryinthuloides, Thraustochytrium, Schizochytrium, Aplanochytrium, Aurantiochytrium, Oblongichytrium, Japonochytrium, Diplophrys , and Ulkenia.
8. The recombinant algal or heterokont cell according to claim 5 , wherein the recombinant algal or heterokont cell is a recombinant algal cell.
9. The recombinant algal cell according to claim 8 , wherein the recombinant algal cell is of a genus selected from the group consisting of Achnanthes, Amphiprora, Amphora, Ankistrodesmus, Asteromonas, Boeketovia, Bolidomonas, Borodinella, Botrydium, Botryococcus, Bracteococcus, Chaetoceros, Carteria, Chlamydomonas, Chlorococcum, Chlorogonium, Chlorella, Chroomonas, Chrysosphaera, Cricosphaera, Crypthecodinium, Cryptomonas, Cyclotella, Desmodesmus, Dunaliella, Elipsoidon, Emiliania, Eremosphaera, Ernodesmius, Euglena, Eustigmatos, Franceia, Fragitaria, Fragilaropsis, Gloeothamnion, Haematococcus, Hantzschia, Heterosigma, Hymenomonas, Isochrysis, Lepocinclis, Micractinium, Monodus, Monoraphidium, Nannochloris, Nannochloropsis, Navicuta, Neochloris, Nephrochloris, Nephroselmis, Nitzschia, Ochromonas, Oedogonium, Oocystis, Ostreococcus, Parachloretta, Parietochloris, Pascheria, Pavlova, Pelagomonas, Phceodactylum, Phagus, Picochlorum, Platymonas, Pteurochrysis, Pleurococcus, Prototheca, Pseudochlorelta, Pseudoneochloris, Pseudostaurastrum, Pyramimonas, Pyrobotrys, Scenedesmus, Schizochlamydella, Skeletonema, Spyrogyra, Stichococcus, Tetrachlorella, Tetraselmis, Thalassiosira, Tribonema, Vaucheria, Viridiella, Vischeria , and Volvox.
10. The recombinant host cell according to claim 9 , wherein the recombinant algal cell is of a genus selected from the group consisting of Cyclotella, Nitzschia, Pavlova, Phceodaclylum , and Thalassiosira.
11. The recombinant algal cell according to claim 9 , wherein the recombinant algal cell is of genus selected from the group consisting of Eustigmatos, Monodus, Nannochloropsis , and Vischeria.
12. The recombinant algal cell according to claim 11 , wherein the recombinant algal cell is a Nannochloropsis cell.
13. A method for producing a biomass or a lipid, comprising culturing the recombinant algal or heterokont cell according to claim 5 and producing the biomass or the lipid therefrom.
14. The method according to claim 13 , further comprising isolating the biomass or the lipid from the culture.
15. The method according to claim 13 , wherein the recombinant algal or heterokont cell is a recombinant algal cell.
16. The method according to claim 15 , wherein the recombinant algal cell is of a genus selected from the group consisting of Achnanthes, Amphiprora, Amphora, Ankistrodesmus, Asteromonas, Boekelovia, Bolidomonas, Borodinella, Botrydium, Botryococcus, Bracteococcus, Chaetoceros, Carteria, Chlamydomonas, Chlorococcum, Chlorogonium, Chlorella, Chroomonas, Chrysosphaera, Cricmphaera, Crypthecodinium, Cryptomonas, Cyclotella, Desmodesmus, Dunaliella, Elipsoidon, Emiliania, Eremosphaera, Ernodesmius, Euglena, Eustigmatos, Franceia, Fragitaria, Fragilaropsis, Gloeothamnion, Haematococcus, I - Iantzschia, Heterosigma, Hymenomonas, Isochrysis, Lepocinclis, Micractinium, Monodus, Monora _ phidium, Nannochloris, Nannochtoropsis, Navicula, Neochloris, Nephrochloris, Nephroselmis, Nitzschia, Ochromonas, Oedogonium, Oocystis, Ostreococcus, Parachlorella, Parietochloris, Pascheria, Pavlova, Pelagomonas, Phceodactylum, Phagus, Picochlorum, Platymonas, Pleurochrysis, Pleurococcus, Prototheca, Pseudochlorella, Pseudoneochloris, Pseudostaurastrum, Pyramimonas, Pyrobotrys, Scenedesmus, Schizochlamydella, Skeletonema, Spyrogyra, Stichococcus, Tetrachlorella, Tetraselmis, Thatassiosira, Tribonema, Vaucheria, Viridielta, Vischeria , and Volvox.
17. The method according claim 16 , wherein said culturing is under photoautotrophic conditions.
18. The isolated or recombinant nucleic acid molecule according to claim 2 , wherein the regulatory element comprises a promoter.
19. The recombinant algal or heterokont cell according to claim 5 , wherein the regulatory element comprises a promoter.