Maize ubiquitin promoters
View Patent ↗The Zea mays c.v. B73 Ubiquitin-1 ( Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. Hi-II.
1. A construct comprising a promoter sequence operably linked to a heterologous nucleic acid sequence, wherein the promoter sequence comprises SEQ ID NO: 2.
2. A construct comprising the complement to the promoter sequence of SEQ ID NO: 2 operably linked to a heterologous nucleic acid sequence.
3. The construct of claim 1 , wherein the operably linked heterologous nucleic acid sequence encodes a heterologous polypeptide.
4. A gene expression cassette comprising the construct of claim 1 operably linked to a 3′-untranslated region.
5. The gene expression cassette of claim 4 , wherein the operably linked heterologous nucleic acid sequence encodes a polypeptide that confers a trait selected from the group consisting of insecticidal resistance, herbicide tolerance, nitrogen use efficiency, water use efficiency, nutritional quality, DNA binding, and selectable marker.
6. A recombinant vector comprising the gene expression cassette of claim 4 .
7. The recombinant vector of claim 6 , wherein the vector is selected from the group consisting of a plasmid vector, a cosmid vector, and a BAC vector.
8. A transgenic cell comprising the construct of claim 1 .
9. The transgenic cell of claim 8 , wherein the transgenic cell is a transgenic plant cell.
10. A transgenic plant comprising the transgenic plant cell of claim 9 .
11. A transgenic seed comprising the construct of claim 1 .
12. The transgenic plant of claim 10 , wherein the transgenic plant is a monocotyledonous transgenic plant or a dicotyledonous transgenic plant.
13. A transgenic plant tissue comprising the construct of claim 1 .
14. The transgenic plant tissue of claim 13 , wherein the transgenic plant tissue is selected from the group consisting of a root, a shoot, a stem and a pollen tissue.
15. The transgenic plant of claim 12 , wherein the transgenic plant is a monocotyledonous transgenic plant.
16. A construct comprising a recombinant polynucleotide, sequence comprising:
a first promoter sequence operably linked to a first heterologous nucleic acid sequence; and
a second promoter sequence operably linked to a second heterologous nucleic acid sequence, wherein said first promoter comprises SEQ ID NO: 1 and the second promoter comprises SEQ ID NO: 2.
17. The transgenic plant of claim 15 , wherein the monocotyledonous transgenic plant is selected from the group consisting of a maize plant, a wheat plant, and a rice plant.
18. The construct of claim 1 , wherein the operably linked heterologous nucleic acid sequence encodes a polypeptide selected from the group consisting of the Yellow Fluorescent Protein from the Phialidium species (PhiYFP) and phosphinothricin-N-acetyl-transferase (PAT).