Antioxidant and antimicrobial wound dressing materials
View Patent ↗A wound dressing material may include a polymeric substrate, a silver salt, and a dyestuff to photostabilize the silver salt. The substrate may include collagen and/or oxidized regenerated cellulose complexed to Ag+, and the dyestuff may, for example, be an aniline or acridine dye. Also disclosed are methods of making such materials, and wound dressings that include such materials.
1. A method of manufacture of an antioxidant wound dressing material, comprising the steps of: dyeing a polymeric substrate material with a dye which stabilizes silver salts against photochemical reduction; and treating the substrate material with a silver salt dissolved or dispersed in water or an organic solvent.
2. The method according to claim 1 , wherein the polymeric substrate material comprises a solid bioabsorbable material.
3. The method according to claim 2 , wherein the solid bioabsorbable material comprises collagens, oxidized celluloses, chitosans, galactomannans, or glycosaminoglycans.
4. The method according to claim 1 , wherein the polymeric substrate material comprises celluloses, alginates, polyacrylates, polyurethanes, or polyamides.
5. The method according to claim 1 , wherein the polymeric substrate comprises an anionic polymer, and said silver salt comprises a salt of Ag + with the anionic polymer.
6. The method according to claim 1 , wherein the dye comprises an antioxidant dyestuff.
7. The method according to claim 6 , wherein the antioxidant dyestuff comprises gentian violet, aniline blue, methylene blue, crystal violet, acriflavine, 9-aminoacridine, acridine yellow, acridine orange, proflavin, quinacrine, brilliant green, trypan blue, trypan red, malachite green, azacrine, methyl violet, methyl orange, methyl yellow, ethyl violet, acid orange, acid yellow, acid blue, acid red, thioflavin, aiphazurine, indigo blue, or methylene green.
8. The method according to claim 1 , wherein the polymeric substrate consists essentially of a mixture of an oxidized cellulose with a collagen and/or a chitosan.
9. The method according to claim 1 , further comprising:
sterilizing the antioxidant wound dressing material; and
packaging the wound dressing material in a microorganism-impermeable container.
10. The method according to claim 1 , wherein the antioxidant wound dressing material has a free radical activity in a diphenylpicrylhydrazyl (DPPH) test for antioxidant activity of at least about 15%.
11. A wound dressing, comprising:
an antioxidant wound dressing material comprising a silver salt and an antioxidant dye; and
a backing sheet configured to be attached to the antioxidant wound dressing material to extend around edges of the antioxidant wound dressing material to form a backing sheet marginal region;
wherein the antioxidant dye is adapted to stabilize silver salts against photochemical reduction; and
the antioxidant dye is present in an amount of from about 0.2 wt. % to about 2 wt. % based on a dry weight of the antioxidant wound dressing material.
12. The wound dressing according to claim 11 , wherein the antioxidant wound dressing material and the backing sheet are sterile and packaged in a microorganism-impermeable container.
13. The wound dressing according to claim 11 , wherein the backing sheet is liquid impermeable.
14. The wound dressing according to claim 11 , wherein the backing sheet has a thickness in a range of approximately 10 to 1,000 μmicrometers.
15. The wound dressing according to claim 11 , wherein the backing sheet has a thickness in a range of approximately 100 to 500 μmicrometers.
16. The wound dressing according to claim 11 , wherein the backing sheet comprises a polymer comprising polyurethanes poly alkoxyalkyl acrylates methacrylates.
17. The wound dressing according to claim 16 , wherein the backing sheet comprises a continuous layer of a high density blocked polyurethane foam.
18. The wound dressing according to claim 11 , further comprising an absorbent layer disposed between the antioxidant wound dressing material and the backing sheet.
19. The wound dressing according to claim 18 , wherein the absorbent layer comprises gauzes, nonwoven fabrics, superabsorbents, or hydrogels.
20. The wound dressing according to claim 18 , wherein the absorbent layer comprises an open-celled hydrophilic polyurethane foam.
21. The wound dressing according to claim 11 , further comprising an adhesive layer disposed between the antioxidant wound dressing material and the backing sheet.
22. The wound dressing according to claim 11 , wherein a removable cover sheet is disposed on a wound-facing surface of the wound dressing.
23. The wound dressing according to claim 11 , wherein the backing sheet is coated with a pressure sensitive adhesive.
24. The wound dressing according to claim 11 , wherein the antioxidant wound dressing material is in the form of a continuous solid sheet, a solid sheet with at least one aperture, a web, a woven fabric, a knitted fabric, a nonwoven fabric, a freeze-dried sponge or a solvent-dried sponge.
25. The wound dressing according to claim 11 , wherein the antioxidant wound dressing material comprises from about 0.01 wt. % to about 5 wt. % of silver, based on a dry weight of the antioxidant wound dressing material.
26. A wound dressing, comprising:
an antioxidant wound dressing material, comprising:
a silver salt, and
a dye which stabilizes the silver salt against photochemical reduction; and
a backing sheet configured to be attached to the antioxidant wound dressing material and to extend around edges of the antioxidant wound dressing material to form a margin.
27. A wound dressing, comprising:
an antioxidant wound dressing material comprising from about 0.01 wt. % to about 5 wt. % of silver, based on a dry weight of the antioxidant wound dressing material; and
a backing sheet configured to be attached to the antioxidant wound dressing material and to extend around edges of the antioxidant wound dressing material to form a margin.
28. A wound dressing, comprising:
an antioxidant wound dressing material comprising a dye adapted to stabilize silver salts against photochemical reduction; and
a backing sheet configured to be attached to the antioxidant wound dressing material and to extend around edges of the antioxidant wound dressing material to form a margin.
29. The wound dressing of claim 28 , wherein the dye is present in an amount of from about 0.2 wt. % to about 2 wt. % based on a dry weight of the antioxidant wound dressing material.
30. The wound dressing of claim 11 , wherein the antioxidant dye comprises gentian violet, aniline blue, methylene blue, crystal violet, acriflavine, 9-aminoacridine, acridine yellow, acridine orange, proflavin, quinacrine, brilliant green, trypan blue, trypan red, malachite green, azacrine, methyl violet, methyl orange, methyl yellow, ethyl violet, acid orange, acid yellow, acid blue, acid red, thioflavin, alphazurine, indigo blue, or methylene green.
31. The wound dressing of claim 11 , wherein the antioxidant dye has an antioxidant activity of at least about 15% in the diphenylpicrylhydrazyl (DPPH) test.
32. The wound dressing of claim 11 , wherein the silver salt comprises silver oxide, silver chromate, silver allantoinate, silver borate, silver glycerolate, silver nitrate, silver acetate, silver chloride, silver sulfate, silver lactate, silver bromide, silver iodide, silver carbonate, silver citrate, silver laurate, silver deoxycholate, silver salicylate, silver p-aminobenzoate, or silver p-aminosalicylate.
33. The wound dressing of claim 26 , wherein the dye comprises gentian violet, aniline blue, methylene blue, crystal violet, acriflavine, 9-aminoacridine, acridine yellow, acridine orange, proflavin, quinacrine, brilliant green, trypan blue, trypan red, malachite green, azacrine, methyl violet, methyl orange, methyl yellow, ethyl violet, acid orange, acid yellow, acid blue, acid red, thioflavin, alphazurine, indigo blue, or methylene green.
34. The wound dressing of claim 26 , wherein the dye has an antioxidant activity of at least about 15% in the diphenylpicrylhydrazyl (DPPH) test.
35. The wound dressing of claim 26 , wherein the silver salt comprises silver oxide, silver chromate, silver allantoinate, silver borate, silver glycerolate, silver nitrate, silver acetate, silver chloride, silver sulfate, silver lactate, silver bromide, silver iodide, silver carbonate, silver citrate, silver laurate, silver deoxycholate, silver salicylate, silver p-aminobenzoate, or silver p-aminosalicylate.
36. The wound dressing of claim 27 , wherein the dye comprises gentian violet, aniline blue, methylene blue, crystal violet, acriflavine, 9-aminoacridine, acridine yellow, acridine orange, proflavin, quinacrine, brilliant green, trypan blue, trypan red, malachite green, azacrine, methyl violet, methyl orange, methyl yellow, ethyl violet, acid orange, acid yellow, acid blue, acid red, thioflavin, alphazurine, indigo blue, or methylene green.
37. The wound dressing of claim 27 , wherein the dye has an antioxidant activity of at least about 15% in the diphenylpicrylhydrazyl (DPPH) test.
38. The wound dressing of claim 27 , wherein the silver salt comprises silver oxide, silver chromate, silver allantoinate, silver borate, silver glycerolate, silver nitrate, silver acetate, silver chloride, silver sulfate, silver lactate, silver bromide, silver iodide, silver carbonate, silver citrate, silver laurate, silver deoxycholate, silver salicylate, silver p-aminobenzoate, or silver p-aminosalicylate.
39. The wound dressing of claim 28 , wherein the dye comprises gentian violet, aniline blue, methylene blue, crystal violet, acriflavine, 9-aminoacridine, acridine yellow, acridine orange, proflavin, quinacrine, brilliant green, trypan blue, trypan red, malachite green, azacrine, methyl violet, methyl orange, methyl yellow, ethyl violet, acid orange, acid yellow, acid blue, acid red, thioflavin, alphazurine, indigo blue, or methylene green.
40. The wound dressing of claim 28 , wherein the dye has an antioxidant activity of at least about 15% in the diphenylpicrylhydrazyl (DPPH) test.
41. The wound dressing of claim 28 , wherein the silver salt comprises silver oxide, silver chromate, silver allantoinate, silver borate, silver glycerolate, silver nitrate, silver acetate, silver chloride, silver sulfate, silver lactate, silver bromide, silver iodide, silver carbonate, silver citrate, silver laurate, silver deoxycholate, silver salicylate, silver p-aminobenzoate, or silver p-aminosalicylate.