Graphical user interface for analysis and comparison of location-specific multiparameter data sets
A graphical user interface on a computer provides for the analysis of location specific data and the presentation of analysis results for visual comparison by a user. Results of the analysis are visually presented as icons subdivided into regions and arranged in such a way that the user is able to associate each icon with a data location. A visual presentation of results in the icons and regions allows a user to visually compare the analysis results in two or more data sets according to location. The graphical user interface further provides for the definition and adjustment of an analysis through the interaction of a user with a graphical representation of the analysis. In some cases, the visual presentation of results tracks the analysis adjustments so the user can visually observe the effects that the adjustments have on the results. A method of interacting with the interface to define an analysis and represent results and a method of presenting two or more data sets using the interface are described. The interface can be used to analyze and visually compare the results of location specific data from a number of sources and is illustrated in a flow cytometry application.
1. A computer system comprising a processor, a display, and a graphical user interface for defining analyses of data sets resulting from cytometric interrogation of particle containing samples in a spatial arrangement of samples and visually representing results of the analyses on the display, where the visual representation enables identification of relationships among different data sets, the interface comprising:
a graphical presentation on the display of the computer system of an analysis that is applied to a data set to produce a set of results, the analysis having at least one parameter that a user controls by interacting with the graphical presentation, wherein the graphical presentation comprises a gate figure in a histogram or dot plot of the set of results, said gate figure defining a range or boundary for identifying events of interrogating and recording light pulses
from particles in the sample, wherein a size, shape, or location of the gate figure is adjustable by the user and the interacting comprises adjusting the size, shape, or location of the gate figure;
a location-specific, multiplexed heat map on the display comprising a visual presentation of the set of results and at least one additional set of results, whereby the user determines the effects of adjusting the gate figure on the relationships among the data sets by viewing the heat map;
the heat comprising a spatial arrangement of icons similar to the spatial arrangement of the particle containing samples such that each of the icons is visually associated with one of the samples; and each of the icons is divided into at least a first region and a second region, the first region of the icon displaying information regarding a first analysis of a data set resulting from cytometric interrogation of a sample visually associated with the icon, and the second region of the icon displaying information regarding a second analysis of the data set resulting from cytometric interrogation of the sample visually associated with the icon.
2. The computer system of claim 1 wherein the samples are particle-containing biological samples.
3. The computer system of claim 1 wherein the samples are interrogated using one of a flow cytometer and a scanning cytometer.
4. The computer system of claim 1 wherein the visual presentation of the set of results tracks the changes in variable analysis parameter.
5. The computer system of claim 4 wherein the variable analysis parameter is changed by adjusting the shape of the gate figure in the graphical presentation.
6. The computer system of claim 5 wherein the gate figure and graphical presentation have one, two or three dimensions.
7. The computer system of claim 4 wherein the variable analysis parameter
is selected by activating buttons on a screen.