Implantable electrochemical biosensor system and method
View Patent ↗Correction for initial variation in thickness of a polymer layer and for changes in the coating thickness that occur after implantation of a biosensor and therefore provides substantial increase in the accuracy and lifetime of implantable sensors is done using a factor derived from the decay of potential.
1. An implantable electrochemical sensor system comprising:
a biosensor comprising an electrode and a polymer layer containing an enzyme;
a controller for first applying a potential (V) to the biosensor when the biosensor is in contact with a solution, wherein the applied potential is sufficient to oxidize or reduce a redox active species in the solution and generate a current, and then switching off the applied potential;
a potentiometer that measures a plurality of V versus time (t) data points after the switching off of the applied potential; and
a data processor that determines the slope (k) of a plot of V versus 1/√t; determines a correction factor for the thickness of the polymer layer from the slope (k), determines an uncorrected measure of analyte in the sample, and corrects the uncorrected measure of analyte in the sample using the correction factor to provide a corrected measure of analyte in the sample.
2. The system of claim 1 , wherein the analyte is glucose and the enzyme is glucose oxidase.
3. The system of claim 2 , wherein the electrode is a platinum electrode.
4. The system of claim 2 , wherein the electrode is a gold electrode.
5. The system of claim 1 , wherein the uncorrected measure of analyte is a current, I meas , measured while potential is applied.
6. The system of claim 5 , wherein the correction factor is 1/k if the redox active species is part of a reversible redox couple, and 1/√k if the redox active species is part of an irreversible redox couple.
7. The system of claim 6 , wherein the biosensor is adapted to be implanted in a living individual, including human individuals, thereby bringing the biosensor into contact with the solution.
8. The system of claim 7 , wherein the analyte is glucose and the enzyme is glucose oxidase.
9. The system of claim 8 , wherein the electrode is platinum.
10. The system claim 8 , wherein the electrode is gold.
11. The system of claim 7 , wherein, for a particular determination of analyte, the potential is applied to the biosensor for a period of 60 seconds or less.
12. The system of claim 11 , wherein the V versus t data points are collected during a period of time that is 5 seconds or less after switching off of the applied potential.