Assays for single molecule detection and use thereof
View Patent ↗The invention relates to methods of detecting a genetic variation in a genetic sample from a subject using labeled probes and counting the number of labels in the probes.
1. A method of detecting trisomy in a fetus of a pregnant human subject,
comprising
contacting first and second probe sets to a cell-free DNA sample from the pregnant human subject, wherein
the first probe set comprises a first labeling probe and a first tagging probe,
the second probe set comprises a second labeling probe and a second tagging probe,
the first and second tagging probes comprise a common tagging nucleotide sequence,
hybridizing at least parts of the first and second probe sets to nucleotide molecules located in first and second chromosomes present in the cell-free DNA sample, respectively,
ligating at least parts of the first probe set by ligating the first labeling probe and the first tagging probe to form a first ligated probe set,
ligating at least parts of the second probe set by ligating the second labeling probe and the second tagging probe to form a second ligated probe set,
amplifying (i) the first ligated probe set with first forward and reverse primers, wherein at least one of the first forward and reverse primers comprises a first label and hybridizes to the first labeling probe of the first ligated probe set, and (ii) the second ligated probe set with second forward and reverse primers, wherein at least one of the second forward and reverse primers comprises a second label and hybridizes to the second labeling probe of the second ligated probe set, to form amplified first and second ligated probe sets comprising (i) the first and second labels, respectively, and (ii) an amplified common tagging nucleotide sequence amplified from said common tagging nucleotide sequence, wherein the first and second labels are different,
immobilizing by hybridizing at least a part of the amplified common tagging nucleotide sequence to affinity nucleotide tags immobilized on a substrate, at a density in which the first and second labels of the amplified first and second ligated probe sets are optically resolvable after immobilization, wherein the affinity nucleotide tags comprise a complementary sequence of the at least a part of the amplified common tagging nucleotide sequence,
reading the first and second labels on the substrate in first and second imaging channels that correspond to the first and second labels, respectively,
producing one or more images of the substrate, wherein the first and second labels are optically resolvable in the one or more images,
distinguishing a first optical signal from a single first label from the rest of the optical signals from background and/or multiple first labels by calculating a relative signal and/or signal-to-noise intensity of the first optical signal compared to an intensity of an optical signal from a single first label, and determining whether the optical signal is from a single label,
distinguishing a second optical signal from a single second label from the rest of the optical signals from background and/or multiple second labels by calculating a relative signal and/or signal-to-noise intensity of the second optical signal compared to an intensity of an optical signal from a single second label, and determining whether the optical signal is from a single label,
counting (i) a first number of the first label from said first optical signal from the single first label, wherein the first number corresponds to a number of the amplified first ligated probe set immobilized to the substrate, and (ii) a second number of the second label from said second optical signal from the single second label, wherein the second number corresponds to a number of the amplified second ligated probe set immobilized to the substrate, and
comparing the first and second numbers to determine whether a copy number of the first chromosome is greater than a copy number of the second chromosome, wherein the copy number of the first chromosome greater than the copy number of the second chromosome indicates the presence of trisomy of the first chromosome in the fetus.
2. The method according to claim 1 , wherein the amplifying step comprises contacting an exonuclease to at least a portion of the amplified first and second ligated probe sets that do not have a label at the 5′-end to digest at least one nucleotide from the 5′-end of said at least a portion of the amplified first and second ligated probe sets.
3. The method according to claim 1 , wherein
the 5′-end of at least a portion of the amplified first and second ligated probe sets comprising a label at the 5′-end is protected from exonuclease digestion.
4. The method according to claim 1 , wherein the trisomy is selected from the group consisting of trisomy 13, trisomy 18, and trisomy 21.
5. The method according to claim 1 , wherein
the trisomy is trisomy 21,
the first chromosome is chromosome 21, and
the second chromosome is not the chromosome 21.
6. The method according to claim 1 , wherein
the comparing step comprises obtaining a ratio of the first and second numbers to determine whether the frequency of the nucleotide molecule located in the first or second chromosome is increased or decreased.
7. The method according to claim 1 , wherein the labels are fluorescent dyes.
8. The method according to claim 1 , wherein
the first and second labeling probes are hybridized to at least parts of the nucleotide molecules located in the first and second chromosomes, respectively;
the first and second tagging probes are hybridized to at least other parts of the nucleotide molecules located in the first and second chromosomes, respectively;
the first labeling probe is hybridized to a region in the first chromosome adjacent to where the first tagging probe is hybridized; and
the second labeling probe is hybridized to a region in the second chromosome adjacent to where the second tagging probe is hybridized.
9. The method according to claim 1 , wherein the numbers of the first and second labels immobilized to the substrate are counted simultaneously by a single scanning.
10. The method according to claim 1 , wherein a plurality of the amplified common tagging nucleotide sequence are immobilized to an array of multiple pre-determined locations on a substrate.
11. The method according to claim 1 , wherein a plurality of the amplified common tagging nucleotide sequence are immobilized to the same member of an array on the substrate.
12. The method according to claim 1 , wherein a plurality of the amplified common tagging nucleotide sequence are immobilized to different members of an array on the substrate.
13. The method according to claim 1 , wherein
the trisomy is trisomy 21, the first chromosome is chromosome 21, and the second chromosome is not the chromosome 21;
the labels are fluorescent dyes; and
the amplified first and second ligated probe sets are immobilized to an array of multiple pre-determined locations on a substrate.
14. The method according to claim 1 , further comprising
contacting first and second control probe sets to the cell-free DNA sample from the pregnant human subject, wherein
the first control probe set comprises a first control labeling probe and a first control tagging probe,
the second control probe set comprises a second control labeling probe and a second control tagging probe,
the first and second control tagging probes comprise first and second control tagging nucleotide sequences, respectively,
hybridizing at least parts of the first and second control probe sets to nucleotide molecules located in first and second control chromosomes present in the cell-free DNA sample, respectively,
ligating at least parts of the first control probe set at least by ligating the first control labeling probe and the first control tagging probe to form a first control ligated probe set,
ligating at least parts of the second control probe set at least by ligating the second control labeling probe and the second control tagging probe to form a second control ligated probe set,
amplifying (i) the first control ligated probe set with first control forward and reverse primers, wherein at least one of the first control forward and reverse primers comprises a first control label and hybridizes to the first control labeling probe of the first control ligated probe set, and (ii) the second control ligated probe set with second control forward and reverse primers, wherein at least one of the second control forward and reverse primers comprises a second control label and hybridizes to the second control labeling probe of the second control ligated probe set, to form amplified first and second control ligated probe sets comprising (a) the first and second control labels, respectively, and (b) amplified first and second control tagging nucleotide sequences amplified from said first and second control tagging nucleotide sequences, respectively,
immobilizing at least parts of the amplified first and second control tagging nucleotide sequences of the amplified first and second control ligated probe sets on the substrate, at a density in which the first and second control labels of the amplified first and second control ligated probe sets are optically resolvable after immobilization,
counting, by optical analysis, (i) a first control number of the first control label in the amplified first control ligated probe set immobilized to the substrate, and (ii) a second control number of the second control label in the amplified second control ligated probe set immobilized to the substrate,
wherein the comparing comprises comparing a first ratio of the first and second control numbers to a second ratio of the first and second numbers to determine whether the second ratio is greater than the first ratio, and
the second ratio greater than the first ratio indicates the presence of trisomy of the first chromosome in the fetus.
15. The method according to claim 1 , wherein the common tagging nucleotide sequence has a non-genomic sequence.
16. The method according to claim 1 , wherein the method excludes quenching the first and second labels.
17. The method according to claim 1 , wherein
the first and second forward primers do not include a label and have the same nucleotide sequence; or
the first and second reverse primers do not include a label and have the same nucleotide sequence.
18. The method according to claim 1 , further comprising
calibrating the first and second numbers by distinguishing said first optical signal from the single first label from the rest of the optical signals from background, and distinguishing said second optical signal from the single second label from the rest of the optical signals from background.
19. The method according to claim 14 , wherein
the first and second labels are different from the first and second control labels, respectively.
20. The method according to claim 1 , wherein the method excludes sequencing of the amplified first and second ligated probe sets.
21. The method according to claim 1 , wherein the counting excludes bulk array readout of the first and/or second labels.
22. The method according to claim 13 , wherein
the method excludes sequencing of the amplified first and second ligated probe sets.
23. The method according to claim 13 , wherein the counting excludes bulk array readout of the first and/or second labels.