Disposable chip-type flow cell and flow cytometer using same
The present invention provides an apparatus for analyzing particles in a solution including a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.
1. A particle-analyzing disposable flow cell for detecting light generated from each sample particle in a sample solution which is illuminated with light while the sample solution flows through a flow path in the flow cell,
wherein the flow cell is made from a transparent substrate configured to form a reflection plane, a sample solution reservoir and a sheath solution reservoir are formed on the flow cell, the sheath solution being supplied to both sides of the sample solution so as to flow on both sides of the sample solution, and the flow cell comprises:
a flow path for sample solution introduction;
a pair of flow paths for sheath solution introduction arranged on both sides of the flow path for sample solution introduction;
a joining flow path joining these and flowing a sheath solution on both sides of the sample solution; and
the reflection plane is formed on a portion of an outer side surface of the flow cell substrate and positioned parallel with the joining flow path, wherein the reflection plane gives total reflection of the light advancing from the joining flow path to an outside of a side surface of the joining flow path, in which the light is scattered light and fluorescence generated within the joining flow path of the flow cell, and the reflection plane reflects scattered light and fluorescence toward a top surface or a bottom surface of the flow cell.
2. The particle-analyzing disposable flow cell according to claim 1 ,
wherein the reflection plane is formed as an inclined surface at about 45° to the surface of the substrate beside the joining flow path, and reflects the light which is generated in the joining flow path and advances within the substrate in an in-plane direction, toward the top surface or the bottom surface of the flow cell.
3. The particle-analyzing disposable flow cell according to claim 1 ,
wherein the thickness of a region to be illuminated in the flow cell is about half of a periphery region thereof, and wherein the flow cell is made from resin.
4. The particle-analyzing disposable flow cell according to claim 1 ,
wherein the flow cell has a flow path and reservoirs on an upstream side and a downstream side of the flow path on the substrate, and the solution flowing through the flow cell does not contact with parts other than the system consisting of an upstream reservoir, the flow path, and a downstream reservoir.
5. A disposable flow cell for detecting light generated by a sample particle in a sample solution which is illuminated with light while the sample solution flows through a flow path in the flow cell,
wherein a sample solution reservoir and a sheath solution reservoir are formed on the flow cell, the sheath solution being supplied to both sides of the sample solution so as to flow on both sides of the sample solution, and the flow cell comprises:
a flow path for sample solution introduction;
a pair of flow paths for sheath solution introduction arranged on both sides of the flow path for sample solution introduction;
a joining flow path joining these and flowing a sheath solution on both sides of the sample solution; and
the flow cell has a flow path formed in a transparent substrate, the transparent substrate configured to form a light reflection plane at an interface between a top flat surface or a bottom flat surface of the substrate and an atmospheric gas, or a side surface of a groove configuration formed in the top or bottom flat surface of the substrate, and directs the light which is generated in the joining flow path and advances within the substrate in an in-plane direction to a specified outside surface of the flow cell.
6. The disposable flow cell according to claim 5 ,
wherein the thickness of a region to be illuminated in the flow cell is about half of a periphery region thereof, and wherein the flow cell is made from resin.
7. The disposable flow cell according to claim 5 ,
wherein the flow cell has a flow path and reservoirs on an upstream side and a downstream side of the flow path on the substrate, and the solution flowing through the flow cell does not contact with parts other than the system consisting of a upstream reservoir, the flow path, and a downstream reservoir.