Neisseria meningitidis compositions and methods thereof
View Patent ↗In one aspect, the invention relates to an isolated polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 71. In another aspect, the invention relates to an immunogenic composition including an isolated non-lipidated, non-pyruvylated ORF2086 polypeptide from Neisseria meningitidis serogroup B, and at least one conjugated capsular saccharide from a meningococcal serogroup.
1. An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 76.
2. The isolated polypeptide according to claim 1 , wherein the polypeptide is lipidated.
3. The isolated polypeptide according to claim 1 , wherein the polypeptide is non-lipidated.
4. The isolated polypeptide according to claim 1 , wherein the polypeptide is immunogenic.
5. An isolated polypeptide comprising the sequence set forth in SEQ ID NO: 76, wherein the cysteine at position 1 is deleted.
6. The isolated polypeptide according to claim 5 , wherein the polypeptide does not exhibit a mass shift of about +70 Da compared to the corresponding non-lipidated polypeptide as measured by mass spectrometry.
7. An isolated polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 76.
8. The isolated polypeptide according to claim 7 , wherein the polypeptide is lipidated.
9. The isolated polypeptide according to claim 7 , wherein the polypeptide is non-lipidated.
10. The isolated polypeptide according to claim 7 , wherein the polypeptide is immunogenic.
11. An immunogenic composition comprising the polypeptide as in any one of claims 1 - 10 .
12. The composition of claim 11 , further comprising an adjuvant.